The Investigator STAR Lyse&Prep Kit reproducibly automate purification of genomic DNA, encountered in forensic and human identity applications. The purification procedure is efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis. It is designed to ensure safe and reproducible handling of precious samples, and comprises 4 steps: lyse, bind, wash and elute.
Since the type of samples that can be processed using the Investigator STAR Lyse&Prep Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions, in the presence of proteinase K and Buffer ATL, in total volumes of either 300 μl (intended for routine use) or 500 μl (facilitating the use of the Investigator Lyse&Spin Basket Kit and also processing of larger samples).
Magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles, in a format designed specifically for open DNA extraction platforms. DNA is isolated from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet. The DNA is then efficiently washed and eluted in modified TE buffer (Buffer ATE).