QIAzol Lysis Reagent

For efficient lysis of fatty and standard tissues before RNA isolation

Features

  • Optimized lysis conditions to purify RNA for gene expression analysis
  • High yields of RNA from fatty tissues
  • Easy-to-follow protocol for lysis and homogenization
  • Integration with RNeasy cleanup to prevent phenol carryover
  • Compatibility with a variety of tissue types
QIAzol Lysis Reagent (200ml)

Cat. No. / ID: 79306

200 ml QIAzol Lysis Reagent
The QIAzol Lysis Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

QIAzol Lysis Reagent is optimized for lysis of fatty tissues. The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation.

Performance

Organic extraction of tissues with QIAzol effectively removes genomic DNA and improves the efficiency of extracted RNA in downstream applications. QIAzol is optimized for lysis of fatty tissues, allowing recovery of high-quality RNA from adipose tissue, especially when used in combination with RNeasy silica membrane technology (see figure " High-quality RNA").
See figures

Principle

Phenol/guanidine-based QIAzol Lysis Reagent can be used to lyse all classes of tissues but is optimized for lysis of fatty tissues, such as brain and adipose tissues. The combination of organic extraction and chaotropic disruption contributes to efficient lysis and higher yields of total RNA. The organic extraction step removes both proteins and DNA, improving the efficiency of further purification steps, for example, precipitation or spin columns. QIAGEN provides several solutions for efficient RNA purification, which integrate QIAzol lysis and RNeasy silica membrane purification technologies in single, streamlined protocols (e.g., RNeasy Plus Universal Kits for any type of tissue).

Procedure

The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation. Tissue samples are homogenized in QIAzol Lysis Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase while DNA partitions to the interphase and proteins to the lower, organic phase. RNA is precipitated from the aqueous phase by addition of isopropanol. The pellet is then washed with ethanol and redissolved in RNase-free water. For high performance in downstream applications, subsequent purification of the RNA is recommended using the RNeasy MinElute Cleanup Kit to remove any contaminating phenol and to concentrate the RNA.

Applications

Phenol/guanidine-based QIAzol Lysis Reagent is suitable for all tissues and is optimized for lysis of fatty tissues, such as brain and adipose tissues. We recommend purification of the RNA product with an RNeasy Plus Universal Kit, or the RNeasy MinElute Cleanup Kit, for high performance in any downstream application.

Supporting data and figures

FAQ

What is the composition of QIAzol? What is the color of this reagent?
For proprietary reasons, the exact composition of QIAzol is confidential.  However, it does contain phenol and thiocyanate compounds.  It is pink in color.
FAQ ID - 3355
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
Yes. Depending on the starting material the aqueous phase might be colored. It will not harm the RNA extraction procedure. It may be a hint at overloading.
FAQ ID -534
Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
Yes, please follow the protocol in the RNAprotect Bacteria Reagent Handbook in Appendix D:  Stabilization and Purification of Bacterial RNA Using RNAprotect Bacteria Reagent, the RNeasy Lipid Tissue Mini Kit, and the TissueLyser.
FAQ ID -1020
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
In general, we recommend using Molecular Biology or higher grade chloroform, which should not contain isoamyl alcohol.
FAQ ID -2344
Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

Yes, we have the following protocols:

  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
  • Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).
FAQ ID -1550
What is the composition of Buffer RWT?
The exact composition of Buffer RWT is confidential. Buffer RWT is a proprietary component of, for example, the miRNeasy Mini Kit and the RNeasy Plus Universal Kit. Guanidine salt and ethanol are important ingredients in Buffer RWT. Ethanol is added by the user prior to the first use of the kit. Buffer RWT is a stringent washing buffer used after preclearing the sample with QIAzol Lysis Reagent, especially if isolation of small RNAs, for example, microRNAs or RNAs from formalin-fixed tissue, is desired
FAQ ID -2798
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
In general we recommend to use Molecular Biology or higher grade chloroform.
FAQ ID -516
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
The RNeasy Lipid Tissue Kits have been designed for the optimal lysis of tissues rich in fat, such as brain and adipose tissues. Lysis and homogenization is performed using QIAzol, a phenol/guanidine-based lysis reagent. The RNA is then purified from the aqueous fraction using a RNeasy spin column. The RNeasy Lipid Tissue Kit combines optimal lysis of fatty tissues with silica membrane technology, resulting in high-quality total RNA.
FAQ ID -468
Can I homogenize samples in QIAzol using the QIAshredder?
Yes
FAQ ID -501
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
Yes, we recommend using the EZ1 RNA Universal Tissue Kit, containing the QIAzol Lysis Reagent, for purification of total RNA from any type of animal and human tissue on the BioRobot EZ1.
FAQ ID -573