QIAwave Plasmid Miniprep Kit

The QIAwave Plasmid Miniprep Kit is an eco-friendlier version of our standard QIAprep Spin Miniprep Kit. The QIAwave Kit uses 15% less plastic and 15% less cardboard than our standard kit and offers waste tubes made 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by 48–80% per bottle. To save paper, there are no printed protocols in the kit. Instead, you can download the protocols you need from the resources list or by simply scanning the QR code inside the kit box lid.

So, while the kit packaging and components of our QIAwave Kit may look different, it’s as easy to use as our standard kit, and the chemistry and performance are identical.

In partnership with My Green Lab, we've also assessed the environmental impact of this kit. My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. These include:

• manufacturing
• responsible chemical management
• sustainable content within products and packaging materials
• disposal of the packaging at the end of life

Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact.

You can view the ACT labels for this kit in the Resources section below.

The QIAwave Plasmid Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA to use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. You can also achieve even higher yields (up to 30 μg) using the High-Yield Supplementary Protocol. For optimal results, we recommend using this kit together with the QIAvac 24 Plus. And if you have too many samples to prep manually, you can automate your sample extraction on the QIAcube Connect.

The performance between our QIAwave Plasmid Miniprep Kit and QIAprep Spin Miniprep Kit is identical because the chemistry is the same. We’ve also shown that both kits outperform competitors’ kits (see figure " QIAwave Kit performance").

The QIAwave Plasmid Miniprep Kit allows you to purify up to 20 μg molecular biology grade plasmid DNA or cosmid DNA to use in routine molecular biology applications such as PCR, sequencing and cloning.

The QIAprep 2.0 Spin Columns are so versatile that you can use them in microcentrifuges, on vacuum manifolds or on the QIAcube Connect (see figures "QIAprep 2.0 Spin Column handling options  A,  D, and  E"). Th vacuum procedure makes handling simpler and sample processing faster. The QIAprep 2.0 Spin Columns can also be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. You can also fully automate the extraction protocol using the QIAcube Connect.

Purified DNA can be used in restriction digestion (see figure "Complete digestion with various restriction enzymes").

The QIAprep 2.0 Spin Columns contain a unique silica membrane that binds up to 20 μg DNA in the presence of a high concentration of chaotropic salt and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time-consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep 2.0 Spin Columns is immediately ready to use – there is no need to precipitate, concentrate, or desalt.

DNA plasmid purification using the QIAwave Plasmid Miniprep follows a simple bind-wash-elute procedure (see flowchart " QIAwave plasmid Miniprep procedure").

1. Lyse bacterial cultures and clear the lysates using centrifugation.

2. Add the cleared lysates to the QIAprep 2.0 Spin Columns. At this point, the plasmid DNA adsorbs to the silica membrane and impurities are washed away.

3. The pure DNA is then eluted in a small volume of elution buffer or water.

In addition to plasmid purification from E. coli, the QIAwave Plasmid Mini Kit can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact our Technical Services Team or your local distributor if you need protocols for these applications.

QIAwave buffers come as concentrates that you can easily reconstitute by adding water and/or ethanol; please check the handbook for details. QIAwave QIAprep 2.0 Spin Columns and Waste Tubes come in individual bags and need to be preassembled before you start the protocol. This takes a little extra time, but it does reduce plastic waste.

If you have too many samples to process manually, you can automate the purification process using the DNeasy mini spin prep columns on the QIAcube Connect.

The QIAwave Plasmid Miniprep Kit provides reproducible yields of high-purity DNA suitable for use in most applications, including:

• PCR
• Restriction digestion
• Ligation and transformation
• Sequencing
• Screening