PCR/qPCR/dPCR
特異的かつ高感度、堅牢なPCRを確実に
遺伝子増幅を行う際に、実験の成功はスループット、アッセイ感度、テンプレートの難易度に起因しない反応のロバストネス、データ正規化の信頼性など、いくつかの重要な要素に左右される可能性があります。使用するPCRメソッドに関連する特定の課題を克服することが必要になる場合もあります。
当社は、お客様がどのメソッドを選択しようと、エンドポイントPCR、qPCR、dPCR用に最適化された豊富なソリューションを提供します。当社のキット、アッセイ、装置、および付属品は、お客様がターゲットを確実に増幅および検出し、ほぼすべてのアプリケーションのワークフローを合理化するのに役立ちます。
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
閲覧 PCR/qPCR/dPCR
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134個の製品が見つかりました
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAcuity Digital PCR System
ナノプレートを使用するデジタルPCRアプリケーション向け
QIAcuity OneStep Advanced Probe Kit
QIAcuityデジタルPCR装置でのワンステップRT-PCR用
QuantiTect PCR Kits
qPCRとSYBR Green I を使用する2ステップqRT-PCRの場合、また、qPCRと2ステップqRT-PCRさらにマルチプレックスqPCRと配列特異的プローブを使用する2ステップマルチプレックスqRT-PCRの場合
QuantiTect RT-PCR Kit
SYBR® Green Iを用いる1ステップqRT-PCR、遺伝子発現分析用の配列特異的プローブを用いる1ステップqRT-PCRおよびマルチプレックスqRT-PCR用
QuantiNova PCR Kits
高感度、高特異的、超高速のプローブベースのreal-time PCRとマルチプレックスPCR、およびSYBR GreenベースのqPCRを使用する比類のない結果を得るため
デジタルPCR用miRCURY LNA miRNA PCR Assays
QIAcuity EvaGreenベースのdPCRを使用する極めて高感度かつ特異的なmiRNAの定量用
QuantiNova RT-PCR Kits
遺伝子発現分析用の配列特異的プローブを使用する感度と特異性の高い1ステップqRT-PCRとマルチプレックスqRT-PCR、またはSYBR® Green Iを使用する1ステップqPCR
Custom Microbial DNA qPCR Arrays
For real-time PCR-based, customized microbial identification or profiling
dPCR Microbial DNA Detection Assay
細菌、真菌、寄生虫、ウイルス、抗生物質耐性または毒性因子遺伝子など、微生物ターゲットのデジタルPCR検出用
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QIAcuity High Multiplex Probe PCR Kit
難しいサンプルおよび5ターゲットを超えるdPCRマルチプレックスに対応
FastLane Cell RT-PCR Kits
For SYBR® Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
QIAcuity Cell and Gene Therapy (CGT) dPCR Assay
QIAcuity Digital PCR Systemでベクターゲノム力価定量およびベクターコピー数の断定を高精度、高再現性、および高速で実現
QIAcuity Residual DNA Quantification and Sizing Kits
For quantification and sizing of host cell DNA with higher accuracy and precision using the QIAcuity Digital PCR System
QIAcuity OneStep Advanced EG Kit
For dye-based one-step RT-PCR on the QIAcuity digital PCR instruments
デジタルPCR用miRCURY LNA miRNA Custom PCR Assays
QIAcuity EvaGreenベースのdPCRを使用する極めて高感度かつ特異的なmiRNAの定量用
Q-Solution Kit
このキットには、より困難なセットアップのPCR反応からより良い結果を得ることができるように、Q-SolutionとMgCl2が含まれています。
QIAcuity Mycoplasma Quant Kit and Standards
For digital PCR quantification and detection of mycoplasma contamination using the QIAcuity Digital PCR System
QIAcuity UCP Probe PCR Kit
QIAcuityデジタルPCR装置で使用するウルトラクリーン生産のキット
dPCR Copy Number Variation（CNV）Probe Assays
シングルプレックスまたはマルチプレックスdPCRによるコピー数多型とコピー数異常の遺伝子座特異的解析用
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
VeraSeq™ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
QIAcuity mericon Food Testing Kits
For digital PCR authentication of food and animal feed using QIAcuity
dPCR PanCancer Kits
For detection of multiple hallmark mutations in cancer-relevant genes using the QIAcuity digital PCR instruments
VeraSeq™ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR
2x HiFi PCR Master Mix
A high efficiency, high-fidelity and low bias PCR master mix for NGS library amplification.
VeraSeq™ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
mericon Ingredient Authentication Assay
For real-time PCR authentication of food and animal feed ingredients
QIAcuity Nanoplatesおよびアクセサリー
QIAcuityデジタルPCR装置で使用
QIAGEN OneStep RT-PCR Kit
高感度で特異的な1ステップRT-PCR
QIAGEN Multiplex PCR Kit
至適化なしに特異性および感度の高いマルチプレックスPCR
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