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Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -2926
Yes. Samples have to be completely lysed and homogenized prior to automated processing on the
QIAsymphony SP platform
using the
QIAsymphony DSP DNA Mini Kit
. Tissue samples are lysed by proteinase K digestion. FFPE tissue samples are deparaffinized with
Deparaffinization Solution
or xylene/ethanol and lysed by proteinase K digestion. Insoluble material is pelleted by centrifugation. Lysates are placed on the QIAsymphony SP for purification of DNA (
see protocol sheets for details
).
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