AllPrep RNA/Protein Kit

For simultaneous purification of RNA and protein from cells

Features

  • High-quality RNA and protein from the same sample
  • Rapid purification with spin-column–based protocol
  • Efficient sample preparation using less cell cultures
  • High-performance RNA and native protein
AllPrep RNA/Protein Kit (50)

Cat. No. / ID: 80404

50 AllPrep Mini Spin Columns, 50 RNeasy Mini Spin Columns, 50 Protein Cleanup Mini Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers
The AllPrep RNA/Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The AllPrep RNA/Protein Kit allows the simultaneous purification of total RNA and native protein from the same cultured cell sample.

Performance

The AllPrep RNA/Protein Kit is designed to purify total RNA and native protein simultaneously from the same sample of cultured cells. Analysis of purified RNA from a sample of cells on the Agilent 2100 Bioanalyzer typically gives RIN values of 10, indicating intact RNA. The native protein purified from the same cell sample contains all cellular proteins (see figure “ Highly intact RNA and high-quality protein from the same cell sample”). RNA purified with the AllPrep RNA/Protein Kit is suitable for real-time RT-PCR (see figure " Sensitivity in real-time RT-PCR") and purified cellular proteins demonstrate functionality in downstream enzyme assays (see figure " Purification of functional protein").
See figures

Principle

The AllPrep RNA/Protein Kit is designed to purify total RNA and native protein simultaneously from the same sample of cultured cells. For experiments to validate siRNA-mediated knockdown at the mRNA and protein levels, the kit streamlines sample preparation and allows the number of cell cultures to be significantly reduced. In addition, knockdown analysis is more reliable since RNA and protein come from the same sample.The AllPrep RNA/Protein Kit is part of QIAGEN’s range of closely integrated products for RNAi and gene expression analysis. These products provide highly reproducible and sensitive results. Products include optimized PCR master mixes, ready-to-run assays, siRNAs, and transfection reagents. Standardization of RNAi and gene expression analysis workflows by these products have contributed to success in many areas of biomedical research.

Procedure

The spin-column–based procedure enables purification of RNA and protein from a single cell sample in less than 40 minutes (see flowchart " AllPrep RNA/Protein procedure"). A method is available for simultaneous purification of DNA, RNA, and protein (suitable for western blotting, not for enzyme assays) from cultured cells and tissues using the AllPrep DNA/RNA/Protein Kit.
See figures

Applications

The AllPrep RNA/Protein Kit allows purification of RNA and protein from a single cell culture. The purified RNA and protein provide high performance in downstream applications, such as real-time RT-PCR, western blotting, and enzyme assays. (Note: The functionality of any protein of interest in an assay has to be tested according to its properties.)

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsPCR, real-time PCR, blotting, microarray
elutionvolume30–50 µl
formatSpin column
processingManual (centrifugation)
technologySilica technology
timeperrunorperprep40 minutes
sampleamount700 µl
mainsampletypeCultured cells
purificationoftotalrnamirnapolyamrnadnaorproteinRNA, native protein

Resources

Kit Handbooks (2)
For simultaneous purification of total RNA and protein from the same animal cell sample
Brochures & Guides (2)
Quick-Start Protocols (1)

FAQ

What is the composition of Buffer APL?
As this is a proprietary reagent, the exact composition of Buffer APL is confidential. Buffer APL is an important component of the AllPrep RNA/Protein Kit. 
FAQ ID -2803
Can the AllPrep RNA/Protein Kit be used with tissue?

No. The AllPrep RNA/Protein Kit has been optimized for cultured cells. If you would like to recover RNA and protein from tissue samples, please follow our Supplementary Protocol 'Acetone precipitation of protein from Buffer RLT or Buffer RLT Plus lysates', (RY22).

FAQ ID -1095
What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?

The maximum number of cells that can be used with the AllPrep RNA/Protein Kit is 6 x 105 cells per preparation. Using larger cell numbers may reduce RNA as well as protein yield and quality.

 

FAQ ID -1208