For instructions regarding sample submission and shipping, please contact QIAGEN Technical Services. Samples accepted are cells or tissue or total DNA. For cells and tissue, a DNA isolation service is available.
For all samples
Please note the minimum amount of starting materials required (see below for requirements for each sample type). If you have less than the minimum amount or your previously isolated samples have been stored differently than we recommend, please contact QIAGEN Technical Services before submitting your samples.
Obtain a sample submission form from QIAGEN Technical Services.
Complete the form and include it with the sample shipment.
Include the amount of material and its storage conditions.
Sign and date the form.
Ship according to the instructions for your sample type.
Provide at least 0.5 million cells for each sample, and specify the number of cells on the submission form. Snap freeze the cell pellets in liquid nitrogen, store samples frozen at –80°C, and ship on dry ice.
Provide at least 50 mg of tissue for each sample, and specify the weight of the tissue on the submission form. Snap freeze tissues in liquid nitrogen, store samples frozen at –80°C, and ship on dry ice.
Purified genomic DNA
Purified genomic DNA is required for methylation analysis. A genomic DNA isolation service is currently also available, please inquire.
Genomic DNA isolated using a standard genomic DNA isolation kit, such as the DNeasy Blood & Tissue Kit, which is compatible with methylation profiling. We recommend 2 µg DNA per sample (in DNase-free water or TE buffer) at a concentration ≥30 ng/µl. A260/A280 ratio of ≥1.7 and A260/A230 ratio of ≥1.7 is required. Customers should check sample integrity by agarose gel electrophoresis before sending samples to QIAGEN. Sample gel pictures, if available, should accompany the submitted samples.
Samples should also be accompanied by a sample submission sheet. Excel format is preferred for sample submission, and the spreadsheet should contain the following information: sample ID, concentration, A260/A280, A260/A230, total volume, total DNA amount.
Genomic DNA isolation service (optional)
Sample preparation and real-time PCR detection with EpiTect Methyl II PCR Arrays
Analysis of the DNA methylation status of 24 or 96 genes simultaneously
Data in an electronic form can be downloaded from a secure FTP website or delivered via email. A complete data and experiment report includes:
- Raw CT values
- ΔCT, for calibrator and samples against endogenous control
- Excel spreadsheet