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QIAseq miRNA Library QC PCR Panel and Assays

For evaluating RNA sample quality prior to miRNA/small RNA NGS library preparation and for assessing NGS performance post-sequencing
  • Unique qPCR-based sample QC of miRNA/small RNA samples prior to NGS
  • Essential for challenging samples with low RNA content, such as biofluids
  • LNA miRNA PCR Assays in ready-to-use PCR panels
  • Compatible with all major qPCR instruments
  • Comprehensive set of 52 RNA spike-ins, spanning a wide range of concentrations
  • Thorough post-sequencing assessment of NGS linearity and reproducibility

QIAseq miRNA Library QC PCR Assays and Arrays enable rigorous quality control of both sample quality before NGS library preparation as well as NGS performance post-sequencing. Amplification of the QIAseq miRNA Library QC Spike-ins – a comprehensive set of spike-ins added during RNA isolation or to isolated RNA – allows assessment of the reproducibility and linearity of the NGS reads mapped to these exogenous sequences, ensuring optimal miRNA sequencing results.

Cat No./ID: 331535
QIAseq miRNA Library QC Spike-ins
$395.00
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52 QIAseq miRNA Library QC Spike-ins, sufficient for up to 500 samples; nuclease-free water
Cat No./ID: 331541
QIAseq miRNA Library QC PCR Panel
Includes 52 RNA spike-ins, 10x RT enzyme mix, 5x reaction buffer, 10x RT primer mix, UniSp6 RNA spike-in template, ready-to-use PCR Panel in 96- or 384-well plate(s), nuclease-free water
Cat No./ID: 331551
QIAseq miRNA Library QC PCR Assays
$658.00
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Includes 52 RNA Spike-ins, 10x RT Enzyme Mix, 5x Reaction Buffer, 10x RT Primer Mix, UniSP6 RNA Spike-in template and qPCR assays for 103a-3p, 191-5p, UniSp6, 451a, 23a-3p, 30c-5p, UniSp-100 and UniSp-101
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Overview of the QIAseq miRNA Library QC PCR Array Kit.
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Useful for a wide range of sample types.
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A rigorous assessment of sample quality.
The QIAseq miRNA Library QC PCR Array Kit uses a combination of endogenous miRNAs and synthetic RNA Spike-ins detected by LNA qPCR assays to perform a rigorous sample QC. Outlier samples and samples affected by hemolysis (e.g., samples 6 and 9) can be readily identified.
Performance

 

Principle

 

Procedure

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