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AdnaTest OvarianCancer

For the enrichment and molecular characterization of circulating tumor cells (CTCs) from whole human blood
  • Efficient isolation and detection of CTCs from whole blood
  • Molecular characterization of CTCs
  • High specificity and sensitivity
  • Processing of multiple samples in parallel
AdnaTest OvarianCancer allows molecular characterization of CTCs by combining AdnaTest OvarianCancerSelect with AdnaTest OvarianCancerDetect using the Combination of Combinations Principle (COCP). AdnaTest OvarianCancerSelect is a highly specific immunomagnetic cell-selection system for enriching circulating tumor cells from peripheral blood. AdnaTest OvarianCancerDetect allows sensitive analysis of ovarian cancer-associated gene expression in immunomagnetically enriched tumor cells by reverse transcription and PCR.
Cat No./ID: T-1-538-R
AdnaTest OvarianCancerSelect
$1,493.00
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For isolation of CTCs and the subsequent extraction of mRNA from human whole blood for 12 preparations
Cat No./ID: T-1-539-R
AdnaTest OvarianCancerDetect
$1,493.00
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RT-PCR kit for detection of ovarian cancer-associated gene expression in enriched tumor cells
Cat No./ID: T-1-610-R
AdnaTube
$125.00
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12 sample tubes containing EDTA. Use only with anticoagulated blood collected in ACDA blood collection tubes from BD (Becton Dickinson, 8.5 ml)
Cat No./ID: T-1-700
AdnaMag-L
$1,143.00
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For 8 tubes, 15 ml
Cat No./ID: T-1-800
AdnaMag-S
$1,143.00
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For 8 tubes, 1.5 ml

AdnaTest OvarianCancer are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers
In a second step the enriched cells are examined by RT-PCR for tumor-associated expression patterns. The mRNA strands are reverse transcribed into cDNA. Subsequently, several tumor-associated markers can be amplified using multiplex PCR and visualized.
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AdnaTest OvarianCancerDetect results of multiplex-PCR samples analyzed with an Agilent 2100 Bioanalyzer
The first lane shows the DNA size standard (DNA-Ladder). Sample 1 is positive for GA733-2, Muc-1 and CA125, samples 3, 4 and 5 are positive for GA733-2 and Muc-1, and sample 6 is positive for GA733-2. Sample 2 is negative. Actin is detected in samples 1 to 6. The PCR negative (C-) and positive control are shown in the last two lanes.
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AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated
In the first step, the CTCs in the blood are enriched (AdnaTest Select). This is achieved using antibody-coated magnetic particles (beads). Several antibodies are used, which bind with high specificity and affinity to the corresponding cancer cells. The enriched cells are lysed and subsequently purified several times to extract mRNA.
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AdnaTest workflow
AdnaTest BreastCancerSelect enables the immunomagnetic enrichment of tumor cells via epithelial and tumor-associated antigens. Antibodies against epithelial and tumor-associated antigens are conjugated to magnetic beads (Dynabeads) for labeling of tumor cells in peripheral blood. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S) and are subsequently lysed. The results of the AdnaTest are generate within 8 hours.
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AdnaTest OvarianCancerDetect results of duplex-PCR samples
The first lane shows the DNA size standard (DNA-Ladder). Samples 1 and 3 are positive for ERCC1. Sample 2 is negative. Actin is detected in samples 1 to 3. The PCR negative (C-) and positive control (ERCC1) are shown in the last two lanes.
Performance
AdnaTest OvarianCancer is a highly specific immunomagnetic cell selection system for enriching circulating tumor cells from peripheral blood and includes a highly sensitive RT-PCR for detecting ovarian cancer-specific mRNA markers.
Principle
AdnaTest technology uses an optimized combination of antibodies for cell selection in 5 ml whole blood and a combination of tumor-associated markers for the detection of tumor cells: EpCAM, CA15-3 (Muc1) and presence of Her2 receptors (optional detection of estrogen and progesterone receptors by AdnaTest ER/PR-Detect).
Procedure
The AdnaTest uses a two-step process (Select and Detect) to generate results within 5 hours (see figures “AdnaTest workflow”). AdnaTest OvarianCancerSelect enables the immunomagnetic enrichment of tumor cells from whole blood via epithelial- and tumor-associated antigens. Antibodies against epithelial- and tumor-associated antigens are conjugated to magnetic beads for labeling of tumor cells. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S), lysed and mRNA is then purified from these lysates (see figures “AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated antibodies labeled to magnetic beads”). In the second step, the isolated mRNA is transcribed into cDNA and can be amplified using multiplex PCR (see figures “AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers”). AdnaTest OvarianCancerDetect contains Oligo (dT) 25-coated beads for the isolation of mRNA from the lysate of pre-enriched tumor cells. Reverse transcription results in cDNA that can subsequently be used as template for tumor cell detection and characterization by multiplex PCR. Multiplex PCR provides tumor-associated gene expression profiles for a variety of relevant tumor markers to ensure that the selected cells are cancer cells. The PrimerMix OvarianDetect allows the amplification of three tumor associated antigens and one control gene. The PrimerMix ERCC1-Detect amplifies the excision repair cross-complementing 1 gene (ERCC1) and one control gene. The two primer-mixes generate the following fragments:
PrimerMix OvarianDetect
 Target gene  PCR fragment size (bp)
 GA733-2  395
 Muc-1  299
 CA125  432
 Actin (internal PCR control)  120
PrimerMix ERCC1-Detect
 Target gene  PCR fragment size (bp)
 ERCC-1  357
 Actin (internal PCR control)  120


Importantly, the AdnaTest is an open platform that allows the profiling of additional custom selected mRNA targets for comphrehensive biomarker discovery.
Applications
  • Isolation of CTCs from whole human blood
  • Molecular characterization of CTCs
  • Biomarker discovery
  • mRNA profiling
  • Liquid biopsy
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