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RNeasy 96 Universal Tissue 8000 Kit

For 96-well purification of total RNA from any tissue on the BioRobot Universal System

  • High yields of total RNA from all tissues
  • Streamlined QIAzol lysis and RNeasy 96 purification
  • Pure, high-quality RNA without phenol contamination
  • High-performance RNA for all downstream applications
The RNeasy 96 Universal Tissue 8000 Kit enables total RNA purification in 96-well format from any tissue. Tissues are efficiently lysed and homogenized in QIAzol Lysis Reagent, and high-quality RNA is purified using silica-membrane RNeasy 96 plates. Automated purification is performed using the BioRobot Universal System, which processes up to 96 or 192 samples per run followed by real-time RT-PCR setup. Manual purification is carried out using the related RNeasy 96 Universal Tissue Kit. Tissue samples can be conveniently stabilized using Allprotect Tissue Reagent, and efficiently disrupted using the TissueLyser II. Nonfatty tissues can be stabilized using the RNAlater RNA Stabilization Reagent.
Cat No./ID: 967852
RNeasy 96 Universal Tissue 8000 Kit (12)
$10,867.00
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For 12 x 96 total RNA preps on the BioRobot 8000 or BioRobot Universal System: 12 RNeasy 96 Plates, Collection Microtubes, Elution Microtubes CL, Caps, S-Blocks, QIAzol Lysis Reagent, RNase-Free Reagents and Buffers
The RNeasy 96 Universal Tissue 8000 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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RNeasy 96 Universal Tissue 8000 procedure.
1
High quality total RNA from rat liver and muscle tissue.
Total RNA was purified from the indicated rat tissues using the RNeasy 96 Universal Tissue Kit and analyzed by formaldehyde agarose gel electrophoresis. The starting materials were 25 mg of RNAlater-stabilized liver and muscle tissue.
2
High-quality RNA from skin tissue.
Total RNA was purified from 50 mg rat skin tissue using the RNeasy 96 Universal Tissue Kit and analyzed on the Agilent 2100 Bioanalyzer.
3
High-quality RNA from adipose tissue.
Total RNA was purified from 100 mg rat adipose tissue using the RNeasy 96 Universal Tissue Kit and analyzed on the Agilent 2100 Bioanalyzer.
4
Real-time analysis of high-quality RNA from rat brain.
Total RNA was purified from 50 mg samples of rat brain using the RNeasy 96 Universal Tissue Kit. Twenty-four purified samples, with an average of 90 ng RNA per reaction, were analyzed by real-time RT-PCR on the ABI PRISM 7900HT Sequence Detection System, using the QuantiTect Probe RT-PCR Kit with primers and probe specific for the c-jun gene.
5
High-quality RNA from liver tissue.
Total RNA was purified from 25 mg rat liver tissue using the RNeasy 96 Universal Tissue Kit and analyzed on the Agilent 2100 Bioanalyzer.
6
High-quality RNA from intestine tissue.
Total RNA was purified from 50 mg rat intestine tissue using the RNeasy 96 Universal Tissue Kit and analyzed on the Agilent 2100 Bioanalyzer.
Performance

Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types (see table).

Typical total RNA yields using the RNeasy 96 Universal Tissue Kit
TissueRNA yield* ((µg per 10 mg of tissue)
Kidney 5–40
Liver 15–80
Lung 5–15
Heart 5–25
Muscle 5–35
Brain 5–20
Adipose tissue 0.5–2.5
Spleen 15–100
Intestine 10–60
Skin 2–5
* Yields can vary due to a number of factors, such as species, age, gender, physiological state, etc.

High-quality RNA can be isolated from tissues preserved either by freezing or by stabilization with RNAlater at room temperature (see figure "High-quality total RNA from rat liver and muscle tissue"). Intact RNA purified from difficult-to-lyse fibrous and fatty tissues is suitable for downstream applications (see figures "High-quality RNA from skin / adipose / liver / intestine tissue" and "Real-time analysis of high-quality RNA from rat brain").

 

Principle

The RNeasy 96 Universal Tissue 8000 Kit represents an advanced technology for high-throughput, automated RNA preparation, enabling purification of RNA from any animal or human tissue sample with the RNeasy 96 Universal Tissue procedure. Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types. The RNeasy procedure enriches for RNA >200 bases long, providing a yield of total RNA that does not include 5.8S rRNA, tRNA, and other low-molecular weight RNAs, which make up 15–20% of total cellular RNA.

 

Procedure

The RNeasy 96 Universal Tissue 8000 Kit integrates efficient phenol/guanidine-based lysis and silica-membrane purification with the speed of vacuum processing. Following homogenization and phase separation, the BioRobot 8000 workstation or BioRobot Universal System provides walkaway automation of the procedure, for total RNA purification from all types of animal tissue (see flowchart "RNeasy 96 Universal Tissue 8000 procedure").

Tissue is first efficiently lysed using QIAzol Lysis Reagent and the TissueLyser II. This provides rapid and parallel disruption of tissues and inactivation of RNases to ensure purification of intact RNA. After phase separation by centrifugation, the BioRobot workstation removes the aqueous phase and adds ethanol to provide appropriate binding conditions. The workstation then applies the sample to the wells of the RNeasy 96 Universal Tissue plate, where total RNA binds and contaminants are efficiently washed away. High-performance RNA is then eluted in a small volume of water, ready for use in any downstream application.

Applications

The combination of QIAzol and RNeasy technologies results in highly pure RNA without phenol carryover. RNA purified using the RNeasy 96 Universal Tissue 8000 Kit is suitable for all downstream applications, including real-time RT-PCR.

Features
Specifications
Applications PCR, qPCR, real-time PCR
Elution volume Custom
For automated processing BioRobot 8000 Workstation or BioRobot Universal System
Format 96-well plate
Main sample type Tissue samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 20–80 mg
Technology Silica technology
Yield Varies

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Kit Handbooks (1)
For high-throughput RNA purification from all types of animal tissue, automated on the BioRobot Universal System
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