For automated high-throughput purification of viral RNA and DNA from a variety of samples
Simple and reliable automated processing for cost and time savings
Suitable for many samples, including blood, tissues, swabs and body fluids
Consistent, high yields
Efficient removal of inhibitors and contaminants
Purified nucleic acids ready for analysis by real-time PCR or RT-PCR
The QIAamp 96 Virus QIAcube HT Kit enables simple, automated purification of viral RNA and DNA on the QIAcube HT system. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis.
Plasticware for 480 typical preps on QIAcube HT:
5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
The QIAamp 96 Virus QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Reliable automated purification.
Viral nucleic acids were purified from 200 µl plasma samples spiked with 10,000, 1000 and 100 IU/ml of a typical DNA or RNA virus. Sample processing was automated using either QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol, QIAxtractor with the VX Protocol, or QIAcube with the QIAamp MinElute Virus Spin Kit. Viral nucleic acids were detected using in-house PCR and RT-PCR assays, with 20 µl eluate per reaction on the Rotor-Gene Q. Results show that QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit performs as well as or better than the other methods.
Reliable purification from a range of sample types.
Various sample types were spiked with 20,000 IU of a typical DNA virus. Viral DNA was purified from 200 µl of each sample lysate using the QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol. Purified viral DNA was detected using an in-house PCR assay with 20 µl each eluate per reaction.
High sensitivity of purified viral nucleic acids in PCR and RT-PCR.
Nucleic acids were purified from serial dilutions of plasma samples spiked with a typical RNA or DNA virus. Samples were processed using QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol and were analyzed using in-house PCR and RT-PCR assays. The percentage of positive samples at low virus titers is shown. The resulting 95% probit values were 316.84 IU/ml for the RNA virus and 18.54 IU/ml for the DNA virus.
The QIAamp 96 Virus QIAcube HT Kit enables automated purification of viral RNA and DNA from a broad range of sample types including fresh or frozen tissues, blood and other body fluids. The procedure yields high-quality viral nucleic acids that perform well in downstream PCR and RT-PCR analyses (see figure Reliable purification from a range of sample types).
The QIAamp 96 Virus QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
Number of samples
24–96 samples (to be processed in increments of 8)
Sample input volume
Blood and other body fluids: up to 200 μl (for sample volumes less than 200 μl, add PBS)
Tissues: up to 20 mg tissue
96 samples in approximately 145 minutes
24 samples in approximately 90 minutes
The QIAamp 96 Virus QIAcube HT procedure is fast and simple with the QIAcube HT instrument. Samples are lysed under highly denaturing conditions at room temperature in the presence of QIAGEN proteinase K and Buffer ACL, which together ensure the inactivation of nucleases. Adding Buffer ACB adjusts the binding conditions for the co-purification of DNA and RNA. The lysate is then transferred to a QIAamp 96 plate. During vacuum, nucleic acids are adsorbed onto the silica membranes while contaminants pass through. Three efficient wash steps remove the remaining contaminants and enzyme inhibitors, and nucleic acids are eluted in Buffer AVE. Some samples may require a pretreatment.
The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)