For the isolation of high-quality, pure DNA from biofilm samples
High DNA yields from difficult biofilm samples
Inhibitor Removal Technology provides high-quality DNA for downstream applications
Optimized isolation of pure DNA from all biofilms, including dental plaques and microbial mats
By pairing chemical and mechanical lysis techniques and Inhibitor Removal Technology (IRT), the DNeasy PowerBiofilm Kit overcomes inefficient cell lysis in the most challenging biofilms. IRT removes concentrated inhibitors such as humic acid, metals, salts and pesticides, typically present in biofilms resulting in increased yields of inhibitor-free DNA from all types of biofilms, including microbial mats.
DNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm DNA Isolation Kit.
For the isolation of high-quality, pure DNA from biofilm samples
The DNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Figure 1
DNA was isolated from biofilm collected from a variety of locations. DNA yield was examined via NanoDrop and quality was assessed by running the samples on a 1.2% agarose gel. High quality, high yield DNA was recovered from each biofilm sample.
Figure 2
DNA was extracted with DNeasy PowerBiofilm Kit from stream epilithic biofilms. Stream pebbles were subjected to sonication to remove biofilm, pelleted with centrifugation then resuspended in Buffer BF1 and transferred to the bead tube. Data provided by University of Stirling, UK: A.T. Gildemeister
Figure 3
The Kapa 2G Fast HotStart kit was used for PCR. Primers used were universal for the 16S rRNA gene (StreF/StreR) and generated a 1,243 bp product.
Performance
Features
Specifications
Bead size
0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix
Binding capacity
Up to 20 µg per prep
Format
Silica Spin Filter Tubes
Processing
Bead Beating
Sample size
0.05 - 0.20 g
Sample types
All processed biofilms from cultured single cell layers to microbial mats
Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 96 adapters with different barcodes (pierceable foil seal, allowing usage of defined parts of plate)
