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AllPrep DNA/RNA 96 Kit

For simultaneous purification of DNA and RNA from cells and tissues in 96-well format
  • Processing of 96 samples in less than 60 minutes
  • High-quality DNA and RNA from the same sample
  • Streamlined protocols for 96-well plates
  • Highly standardized workflow
  • Highly reproducible DNA and RNA yields

The AllPrep DNA/RNA 96 Kit is well suited for high-throughput purification of genomic DNA and total RNA from up to 96 samples. DNA and RNA are simultaneously purified from each cell or tissue sample. Since there is no need to divide each sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately.

Cat No./ID: 80311
AllPrep DNA/RNA 96 Kit (4)
$4,122.00
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For 4 x 96 preps: 4 AllPrep 96 DNA Plates, 4 RNeasy 96 Plates, Elution Microtubes CL, Caps, S-Blocks, AirPore Tape Sheets, RNase-Free Reagents and Buffers
The AllPrep DNA/RNA 96 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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AllPrep DNA/RNA procedure.
With the AllPrep DNA/RNA 96 Kit, an AllPrep 96 DNA plate and RNeasy 96 plate are used in the procedure. With the AllPrep DNA/RNA Micro Kit and AllPrep DNA/RNA Mini Kit, spin columns are used in the procedure. 
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Reproducible purification of DNA and RNA in 96-well format.
Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. [A] Purified DNA samples were analyzed on a 0.8% agarose gel. [B] Purified RNA samples were analyzed on a 1.2% formaldehyde agarose gel. M: GelPilot Lambda HindIII Marker.
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High-quality DNA for reliable multiplex PCR analysis.
Genomic DNA was purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. Six targets (195-1150 bp) were amplified by multiplex PCR and then analyzed on the QIAxcel system. Li: liver; Ki: kidney; Lu: lung; In: intestine; Sp: spleen; NTC: no template control (shows upper and lower markers).
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RNA with no genomic DNA contamination.
Total RNA was purified from rat kidney using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (–RT) indicates the absence of genomic DNA.
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AllPrep RNA 96-well plate.

The 96-well RNA purification plate of the AllPrep DNA/RNA 96 Kit.

Performance
AllPrep DNA/RNA 96 Kit achieved efficient purification of high-quality DNA and RNA from different tissue types (see table, and figure "Reproducible purification of DNA and RNA in 96-well format").

High-quality RNA from tissues purified using the AllPrep DNA/RNA 96 Kit
Rat tissueRIN value*
Liver 9.4
Lung 9.5
Spleen 9.6
Thymus 10.0
* Intact RNA is indicated by a RIN value of close to 10 analyzed on the Agilent Bioanalyzer 2100.
There is no need for additional RNase or DNase digestion (see figure "RNA with no genomic DNA contamination") and the purified DNA is suitable for downstream applications (see figure "High-quality DNA for reliable multiplex PCR analysis" ). 

 

 

 

Principle

By allowing the analysis of DNA and RNA from the same sample, the AllPrep DNA/RNA 96 Kit is the ideal tool for sample preparation in genomics and systems biology. Efficient purification of high-quality DNA and RNA from different tissue types is achieved without the need for additional RNase or DNase digestion. Both cultured cells and easy-to-lyse tissues can be processed. The kits form part of QIAGEN's solution for preparing multiple analytes from the same sample.

Procedure

A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart "AllPrep DNA/RNA procedure"). The 96-well purification plates of the kit are for DNA and RNA (see figure "AllPrep RNA 96-well plate"; DNA plate not shown). The plates are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-16 and Plate Rotor 2 x 96) or a combination of vacuum (QIAvac 96) and centrifuge. Both cultured cells and easy-to-lyse tissues can be processed.

The kits form part of QIAGEN's solution for preparing multiple analytes from the same sample. These include Allprotect Tissue Reagent, which stabilizes DNA, RNA, and protein in tissues, and RNAprotect Cell Reagent, which stabilizes DNA and RNA in cells. Both reagents deliver immediate stabilization at room temperature. For tissues, TissueRuptor and TissueLyser systems provide fast disruption at low- to high-throughputs.

Applications

The AllPrep DNA/RNA 96 Kit is the ideal tool for sample preparation in genomics and systems biology. The purified genomic DNA has an average length of 15–30 kb, depending on homogenization conditions, and is well suited for any application, including Southern-, dot-, and, slot-blot analyses; DNA methylation analysis; and PCR and multiplex PCR. The purified total RNA is ready to use in any application, such as RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarray analysis.

Features
Specifications
Applications PCR, real-time PCR, microarray, blotting
Elution volume 50 - 100 µl
Format 96-well plate
Main sample type Cultured cells, easy-to-lyse tissue
Processing Manual (centrifugation and/or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Total RNA, DNA, miRNA (optional)
Sample amount up to 2 x 10e6 cells / up to 10 mg tissue
Technology Silica technology
Time per run or per prep 60 minutes
Yield varies

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Kit Handbooks (2)
動物およびヒトの細胞/組織からゲノムDNAとトータルRNAを96ウェルフォーマットで同時に分離精製
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For simultaneous purification of genomic DNA and total RNA from animal and human cells and tissues in 96-well format
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References
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