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Ni-NTA HisSorb Plates

For assays using 6xHis-tagged biomolecules
  • Enhanced signal-to-noise ratio and reproducibility
  • Convenient multiwell-based assay system
  • Reduced optimization for faster assay development

Ni-NTA HisSorb Plates are 96-well plates whose wells are coated with Ni-NTA. They are used for immobilization of His-tagged proteins in protein interaction assays, such as ELISA.

Cat No./ID: 35061
Ni-NTA HisSorb Plates (5)
$471.00
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5 Ni-NTA-coated, transparent 96-well plates

Ni-NTA HisSorb Plates are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Principle
Ni-NTA HisSorb Plates provide the power of the QIAexpress Ni-NTA System in solid-phase transparent 96-well format for convenient assay and screening of His-tagged biomolecules, even if crude lysates. Ni-NTA moieties, which coat the entire surface of each well, deliver effective, directed immobilization of functionally active His-tagged molecules, leading to more reliable and reproducible assay results and higher signal-to-noise ratio than are possible with other systems.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications

Ni-NTA HisSorb Plates are suitable for a range of applications, including:

Quantitation of His-tagged molecules
ELISA
Hybridoma and antibody screening
Drug discovery
Screening expression clones and engineered enzymes 
Features
Specifications
Epitope detected Histidine residued epitope
Format Plate based
Instrument Manual
Ligand on bead or plate Ligand on plate
Tag 6xHis tag

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Handbooks & Protocols (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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Kit Handbooks (2)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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