RT2 ProfilerTM miRNA Targets PCR Arrays
The RT² Profiler microRNA Targets PCR Arrays are designed to facilitate miRNA functional analysis as a follow up to miRNA expression profiling analyses. While the general function of miRNAs is well-characterized, the complex role played by each miRNA is yet to be completely defined. Functionally validating a biological or mechanistic effect of specific miRNA's expression remains one of the major challenges in miRNA research. Each of these arrays profiles the expression of 84 verified and bioinformatically predicted mRNA target genes of a specific human, mouse, or rat miRNA including other miRNAs in the same seed sequence family. One miRNA can regulate one or more messenger RNA transcripts. Conversely, one mRNA transcript can be regulated by a single or multiple miRNAs. Because they represent gene targets selected based on both miRNA functional studies and bioinformatic prediction algorithms, these PCR Arrays maximize the likelihood of further validating and establishing miRNA target genes whose expression patterns inversely correlate with a miRNA of interest under study.
RT² Profiler microRNA Targets PCR Arrays provide all of the benefits and performance of the RT² Profiler PCR Arrays.
How can these arrays be used to identify miRNA target genes?
Follow up miRNA expression analysis using miScript miRNA PCR Arrays with a miRNA functional study.
A guaranteed system
RT2 Profiler PCR Arrays are tested and optimized in combination with the RT2 SYBR® Green qPCR Mastermixes and the RT2 First Strand Kit. This testing means that RT2 Profiler PCR Array performance is guaranteed when all three of these components are used together.
When using the RT2 First Strand Kit, start with as little as 1 ng or as much as 5 µg of total RNA per sample.
The complete PCR array system demonstrates strong correlations across technical replicates, lots, and instruments with average correlation coefficients >0.99, ensuring reliable detection of differences in expression between biological samples.
The PCR array system, with high-quality input RNA, yields single bands of the predicted size without primer-dimers or other secondary products, therefore providing the highly accurate real-time PCR results.
Uniform PCR amplification efficiency
Uniform PCR amplification efficiency is required for the PCR array technology to allow accurate comparisons of gene expression across all genes and all samples. The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees the high performance of every primer assay on PCR arrays.
RT2 Profiler PCR Arrays are reliable tools for analyzing the expression of a focused panel of genes. Each 96-well plate, 384-well plate or 100-well disc PCR array includes SYBR Green-optimized primer assays for a thoroughly researched panel of relevant, pathway- or disease-focused genes. RT2 Profiler PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests. The high-quality primer design and RT2 SYBR Green qPCR Mastermix formulation enable the PCR array to amplify 96 or 384 different gene-specific products simultaneously under uniform cycling conditions.
This combination provides the RT2 Profiler PCR Array with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. PCR arrays are easy to use in any research laboratory.RT2 Profiler PCR Arrays are sensitive enough for use with RNA prepared from regular samples (0.1–5 µg RNA), FFPE samples, and small samples (1–100 ng RNA).
Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. RT2 Profiler PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments.
Flexible layout and controls
RT2 Profiler PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway, plus 5 housekeeping genes. Each RT2 Profiler PCR Array also includes control elements for:
Easy-to-use data analysis
Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔCT method with normalization of the raw data to either housekeeping genes.
RT2 PCR Profiler Arrays can be used in all areas of biological and medical research, including: