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Custom RT2 lncRNA PCR Arrays

For profiling a customized panel of lncRNAs by real-time PCR

  • Choose from over 25,000 human or 10,000 mouse lncRNAs
  • Ideal for discovery or RNA-seq and microarray verification
  • Available in 384- or 96-well plate and 100-well ring disc formats
  • Combine lncRNA and mRNA for greater insight
Custom RT2 lncRNA PCR Arrays are sets of optimized real-time PCR primer assays on 96-well plates, 384-well plates, or 100-well discs for a panel of genes related to a user's unique research area of interest and include appropriate RNA quality controls.

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Custom RT2 lncRNA PCR Array
96-well plate, 384-well plate, or Rotor-Disc 100 including assays, film, or cap strips
330731 330731 Create and order

Custom RT2 lncRNA PCR Arrays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Principle
Custom RT2 lncRNA PCR Arrays are user-defined panels of RT2 lncRNA qPCR Assays for long noncoding RNAs. Assays are designed using a computer algorithm which has been developed using an in vitro assay to ensure that the resulting primer sequences generate a single PCR product of the predicted size without primer-dimers or off-target amplification in 33 cycles of PCR amplification. The assay also ensures that the amplification efficiency of the primers is 100 +/– 10%. As a result, the algorithm designs highly effective primer sequences for SYBR Green based real-time PCR detection.

For optimal performance, Custom RT2 lncRNA PCR Arrays should be used together with the RT2 First Strand Kit for cDNA synthesis and RT2 SYBR Green Mastermixes for qPCR. These reagents have been formulated and pretested together with RT2 lncRNA PCR Arrays. The RT2 First Strand Kit includes a proprietary genomic DNA elimination step to remove any residual contamination in RNA samples before reverse transcription, thereby eliminating false positive signals. In addition, an artificial RNA control template is also included which can be used to follow the reverse transcription efficiency across samples and monitor against RNase contamination. Each of the real-time instrument-specific RT2 SYBR Green Mastermixes contains SYBR Green and an appropriate reference dye to match the instrumentation available in your laboratory. RT2 SYBR Green Mastermixes are available for all real-time PCR instruments from QIAGEN, Applied Biosystems, Bio-Rad, Stratagene, Eppendorf, Roche, and other major suppliers.
Procedure
Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. Custom RT2 lncRNA PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments.

Flexible layout and controls
Custom RT2 lncRNA PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and is used to monitor the expression of 84 or 370 lncRNA genes related to an individual user's research interests. Five reference genes are included, as well as control elements for:

  • Data normalization
  • Genomic DNA contamination detection
  • RNA sample quality
  • General PCR performance
  • Easy-to-use data analysis
Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔCq method with normalization of the raw data to either housekeeping genes
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