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AdnaTest ColonCancer

For the enrichment and molecular characterization of circulating tumor cells (CTCs) from whole human blood
  • Efficient isolation and detection of CTCs from whole blood
  • Molecular characterization of CTCs
  • High specificity and sensitivity
  • Processing of multiple samples in parallel
AdnaTest ColonCancer allows molecular characterization of CTCs by combining AdnaTest ColonCancerSelect with AdnaTest ColonCancerDetect using the Combination of Combinations Principle (COCP). AdnaTest ColonCancerSelect is a highly specific immunomagnetic cell-selection system for enriching circulating tumor cells from peripheral blood. AdnaTest ColonCancerDetect allows sensitive analysis of colon cancer-associated gene expression in immunomagnetically enriched tumor cells by reverse transcription and PCR.
Cat No./ID: T-1-504-R
AdnaTest ColonCancerSelect
$1,493.00
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For isolation of CTCs and the subsequent extraction of mRNA from human whole blood for 12 preparations
Cat No./ID: T-1-505-R
AdnaTest ColonCancerDetect
$1,493.00
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RT-PCR kit for detection of colon cancer-associated gene expression in enriched tumor cells
Cat No./ID: T-1-610-R
AdnaTube
$125.00
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12 sample tubes containing EDTA. Use only with anticoagulated blood collected in ACDA blood collection tubes from BD (Becton Dickinson, 8.5 ml)
Cat No./ID: T-1-700
AdnaMag-L
$1,143.00
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For 8 tubes, 15 ml
Cat No./ID: T-1-800
AdnaMag-S
$1,143.00
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For 8 tubes, 1.5 ml

AdnaTest ColonCancer are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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AdnaTest ColonCancerDetect results of samples analyzed with an Agilent 2100 Bioanalyzer
The first lane shows the DNA size standard (DNA-Ladder). Sample 1 and 4 are positive for GA733-2 and CEA, samples 2 is negative and sample 3 is positive for GA733-2, CEA and EGFR. Actin is detected in samples 1 to 4. The PCR negative (C-) and positive control (C+) are shown in the last two lanes.
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AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers
In a second step the enriched cells are examined by RT-PCR for tumor-associated expression patterns. The mRNA strands are reverse transcribed into cDNA. Subsequently, several tumor-associated markers can be amplified using multiplex PCR and visualized.
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AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated
In the first step, the CTCs in the blood are enriched (AdnaTest Select). This is achieved using antibody-coated magnetic particles (beads). Several antibodies are used, which bind with high specificity and affinity to the corresponding cancer cells. The enriched cells are lysed and subsequently purified several times to extract mRNA.
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AdnaTest workflow
AdnaTest BreastCancerSelect enables the immunomagnetic enrichment of tumor cells via epithelial and tumor-associated antigens. Antibodies against epithelial and tumor-associated antigens are conjugated to magnetic beads (Dynabeads) for labeling of tumor cells in peripheral blood. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S) and are subsequently lysed. The results of the AdnaTest are generate within 8 hours.
Performance
AdnaTest ColonCancer is a highly specific immunomagnetic cell selection system for enriching circulating tumor cells from peripheral blood and includes a highly sensitive RT-PCR for detecting colon cancer-specific mRNA markers.
Principle
AdnaTest technology uses an optimized combination of antibodies for cell selection in 5 ml whole blood and a combination of tumor-associated markers for the detection of tumor cells: EpCAM, CEA and EGFR.
Procedure
The AdnaTest uses a two-step process (Select and Detect) to generate results within 5 hours (see figures “AdnaTest workflow”). AdnaTest ColonCancerSelect enables the immunomagnetic enrichment of tumor cells from whole blood via epithelial- and tumor-associated antigens. Antibodies against epithelial- and tumor-associated antigens are conjugated to magnetic beads for labeling of tumor cells. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S), lysed and mRNA is then purified from these lysates (see figures “AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated antibodies labeled to magnetic beads.”). In the second step, the isolated mRNA is transcribed into cDNA and can be amplified using multiplex PCR (see figures “AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers”). AdnaTest ColonCancerDetect contains Oligo (dT) 25-coated beads for the isolation of mRNA from the lysate of pre-enriched tumor cells. Reverse transcription results in cDNA that can subsequently be used as template for tumor cell detection and characterization by multiplex PCR. Multiplex PCR provides tumor-associated gene expression profiles for a variety of relevant tumor markers to ensure that the selected cells are cancer cells. The PrimerMix ColonDetect allows amplification of 3 tumor-associated genes and 1 control gene (see table below). The primers generate fragments of the following sizes:
PrimerMix ColonDetect
 Target gene  PCR Fragment Size (bp)
 GA733-2  383
 CEA  231
 EGFR  163
 Actin (internal PCR control)  120

Importantly, the AdnaTest is an open platform that allows the profiling of additional custom selected mRNA targets for comphrehensive biomarker discovery.
Applications
  • Isolation of CTCs from whole human blood
  • Molecular characterization of CTCs
  • Biomarker discovery
  • mRNA profiling
  • Liquid biopsy
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