For effortless DNA and RNA analysis
  • Rapid analysis of up to 96 samples without manual intervention
  • Safety and convenience with ready-to-use gel cartridges
  • Accurate analysis of low concentration nucleic acids with 3–5 bp resolution
  • User-friendly analysis software that supports electronic signatures and records
  • Objective RNA quality measurement with new RNA Integrity Score (RIS)
 
The revolutionary QIAxcel Advanced system replaces traditional, labor-intensive gel analysis of DNA and RNA — streamlining workflows and reducing time to result. The QIAxcel Advanced system fully automates sensitive, high-resolution capillary electrophoresis of up to 96 samples per run. DNA fragment analysis of 12 samples can be performed in as little as 3 minutes. Ready-to-run gel cartridges allow 96 samples to be analyzed with a minimum of hands-on interaction, reducing manual handling errors and eliminating the need for tedious gel preparation. User-friendly QIAxcel ScreenGel software ensures convenient analysis and documentation of data. The QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement of the analyzed samples and allows easy interpretation of sample integrity.

Explore the virtual demo to learn more about the QIAxcel Advanced system.

The QIAxcel Advanced system operates in conjunction with the QIAxcel DNA Kits and QIAxcel RNA QC Kit v2.0.
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QIAxcel Advanced Instrument
Capillary electrophoresis device: includes computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor
9001941
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QIAxcel Advanced System
Capillary electrophoresis device: includes computer, QIAxcel ScreenGel Software, installation, training, and 1-year warranty on parts and labor
9002123
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QIAxcel Advanced Priority Package
Capillary electrophoresis device: includes Priority Package with computer, QIAxcel ScreenGel Software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9002124
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QIAxcel Advanced Priority Package Plus
Capillary electrophoresis device: includes Priority Package Plus with computer, QIAxcel ScreenGel Software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9002125
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QIAxcel ScreenGel Software
Separate license for use of QIAxcel ScreenGel Software on an additional computer
9021163
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QIAxcel ScreenGel Software (10)
Ten licenses for use of QIAxcel ScreenGel Software on additional computers
9021165
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The QIAxcel Advanced System is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Assessment of DNA degraded by sonication.|Reduced run times|Streamlined workflow.|Streamlined RNA analysis using the QIAxcel Advanced System.|RIS indicates RNA integrity.|Accurate, high-throughput genotyping of bacteria.|Analysis of purified RNA.|Sample separation process|Ready-to-run gel cartridges.|Simplified and standardized data collection and analysis.|Easy-to-use software features.|
[A] Agarose gel photo showing gDNA in various states of degradation. Lane 1: Non-degraded gDNA samples; lanes 2 and 3: gDNA samples partially degraded by sonication with 7 and 14 pulses, respectively; lanes 4 and 5: gDNA samples fully degraded with 70 and 105 pulses, respectively. [B] QIAxcel gel photo showing gDNA samples in three states of degradation. 1, 3, and 5 correspond to lanes 1, 3, and 5 in [A]. [C] Electropherograms and a gel photo of gDNA samples from lanes 1, 3, and 5 in [A]. Lane 1: Electropherogram indicates very good quality gDNA (no degradation products before the major peak, long tailing off); lane 3: partially degraded gDNA (some degradation product signals, no tailing off); lane 5: highly degraded gDNA (many degradation product signals, no tailing off).|Nucleic acid separation on the QIAxcel Advanced System, using the QX DNA High Resolution Kit takes less than 10% of the time of conventional high-resolution agarose.||Total RNA purified from Schizosaccharomyces pombe. Results presented as [A] a gel image and [B] a superimposed electropherogram view. Reliable quality control of unlabeled cRNA generated from yeast total RNA. Results presented as [C] a gel image and [D] a superimposed electropherogram view.|Total RNA was extracted from rat liver samples using the RNeasy Plus Mini Kit and subjected to decomposing conditions. Samples of increasing levels of degradation were analyzed on the QIAxcel Advanced and the Agilent 2100 Bioanalyzer platform. The results are depicted in the gel view pictures for the QIAxcel Advanced [A] and the Agilent Bioanalyzer [B]. The electropherogram of selected samples analyzed on the QIAxcel Advanced is shown in [C]. The RIS (QIAxcel Advanced) and RIN (Agilent Bioanalyzer) numbers are listed in the table.|Samples were analyzed on either [A] the QIAxcel Advanced System at the original concentration or [B] by agarose gel electrophoresis with five-fold concentration. Samples 3 [C] and 5 [D] were analyzed by agarose gel electrophoresis at the original concentration (O), with five-fold concentration (C), and at the original concentration on the QIAxcel Advanced (Q). The arrows represent bands originally scored as negative. Data kindly provided by Mutschall and coworkers, Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada.|Electropherogram views of purified total RNA. Data kindly provided by D. van Leenen, Microarray Facility, University Medical Center Utrecht, The Netherlands.|Nucleic acid molecules are size separated by applying a current to a gel-filled capillary, and detected as they migrate toward the positively charged terminus. The signal data pass through a photomultiplier and are converted to an electropherogram and gel image by the QIAxcel ScreenGel Software.|QIAxcel gel cartridges minimize exposure to hazardous chemicals.|QIAxcel ScreenGel software simplifies data analysis.|QIAxcel ScreenGel software simplifies experiment setup and data analysis.|
Performance
Streamlined workflow
The QIAxcel Advanced system not only speeds up DNA and RNA analysis by eliminating slab-gel analysis, but also contributes to streamlining the entire sample purification and analysis workflow. Used in combination with proven QIAGEN end-point PCR kits, the QIAxcel Advanced provides an all-in-one, pretested solution for reliable analysis of PCR fragments, ensuring reproducibility and significant time and cost savings.

One instrument, many applications
The QIAxcel Advanced system is a versatile solution for electrophoresis and offers a broad range of applications. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, total RNA, and cRNA. Unlike other commercially available devices that provide processing of only up to 12 samples at time, the QIAxcel Advanced system allows processing of all 96 samples without manual intervention.

DNA analysis
In genotyping applications, the QIAxcel Advanced system enables greater sizing accuracy and more sensitive detection than conventional agarose gel electrophoresis (see figure Accurate, high-throughput genotyping of bacteria). QIAxcel Advanced also provides a straightforward and effective means for checking the quality of genomic DNA (gDNA) samples prior to molecular screening and downstream assays (see figure Assessment of DNA degraded by sonication).

RNA analysis — objective quality values with the RNA Integrity Score
The new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement for eukaryotic RNA samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.

Such careful monitoring of the initial RNA sample, as well as products generated throughout the entire procedure, is crucial in microarray analysis, since RNA degradation strongly influences the predictive power obtained from the microarray data. The QIAxcel Advanced System is highly suitable for analyzing the integrity of total RNA and size distribution of fragmented or intact cRNA (see figure Streamlined RNA analysis using the QIAxcel system), and the throughput capacity makes it very convenient for laboratories employing 96-well RNA purification technologies that need a fast solution for quality control.

Principle
The QIAxcel Advanced instrument includes an array of light-emitting diodes and micro-optical collectors that latch to capillaries within QIAxcel gel cartridges. Fragments that are migrating through a gel matrix within the capillary pass excitation and detection spots and the signal is transmitted through a photomultiplier tube to the QIAxcel ScreenGel software for data interpretation (see figure Sample separation process).

The high detection sensitivity provided by the QIAxcel Advanced instrument enables robust results even with low concentrations of nucleic acid. With a resolution of 3–5 bp for fragments smaller than 0.5 kb, the QIAxcel Advanced system ensures greater accuracy than slab-gel methods, as well as greater confidence in data interpretation. Sample consumption is less than 0.1 μl per analysis, saving precious samples for further downstream analysis. Hands-free sample loading and self-contained components minimize exposure to hazardous chemicals, such as ethidium bromide (see figure Ready-to-run gel cartridges).

A range of QIAxcel Kits is available for a variety of applications, meeting different resolution and speed requirements (see table below).

QIAxcel Kits
QIAxcel Kit Analyte Size range 100 bp – 500 bp* 500 bp – 1 kb* 1 kb – 5 kb* 5 kb – 10 kb* Run time/
12 samples
QIAxcel DNA High Resolution Kit DNA 15 bp–10 kb 3–5 bp 50 bp 200–500 bp 1–1.5 kb 7–20 min
QIAxcel DNA Screening Kit DNA 15 bp–5 kb 20–50 bp 50–100 bp 500 bp 5 min
QIAxcel DNA Fast Analysis Kit DNA 15 bp–3 kb 50–100 bp 100–250 bp 250 bp – 1 kb 3–5 min
QIAxcel RNA QC Kit v2.0 RNA 15 b–6 kb 10 min
* Best resolution. Run time depends on method used. Best resolution between 1–3 kb.
Procedure
Just a few, simple steps are required to operate the QIAxcel Advanced system for analysis of DNA and RNA samples: load the gel cartridge, fill and load the buffer tray, load the samples in 96-well plates or in PCR tubes or strips, select the process profile to be used — and go! Tedious gel preparation and extensive user training are eliminated, streamlining the workflow and facilitating integration of the system into the daily lab routine. Within minutes of starting a run, the first results appear in real time on the computer screen (see figure Reduced run times).
Applications

The QIAxcel Advanced System offers a broad range of optimized applications for DNA and RNA analysis and is highly suited for research in academic as well as pharmaceutical, biotechnology, and biomedical laboratories. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, synthesized oligonucleotides, total RNA, and cRNA.

The QIAxcel Advanced System has been used for applications such as:

Bacterial genotyping
PCR fragment analysis
Pre-sequencing testing
Mutation detection
Detection of food allergens
Quality control of genomic DNA
Total RNA and cRNA QC for RT-PCR and microarray analysis
Software
QIAxcel ScreenGel software provides a new level of convenience
QIAxcel ScreenGel software, specifically developed for use with the QIAxcel Advanced system, is a powerful and user-friendly tool for data collection and analysis. Interactive tools simplify analysis, facilitate rapid data interpretation, and provide flexibility with data and results displayed in both electropherogram and gel image formats. Results can be viewed individually or displayed as overlay views for sample and data comparison. All-in-one analysis for multiple data sets simplifies evaluation. A unique algorithm calculates and generates a tabular display of a variety of peak properties, including number of peaks as well as the size, height, width, and area of each peak. Comprehensive data reports can be easily generated and saved or exported to meet individual documentation needs. Complete process profiles for standardized sample processing — from running samples to data analysis, generating reports and exporting data — minimize the need for additional training for users (see figure Simplified and standardized data collection and analysis).

Easy setup and start
The Process Wizard simplifies experiment startup (see figure Easy-to-use software features). This convenient feature enables guided setup and allows run parameters to be defined and DNA size markers to be selected. Reagent lot number information can be included. Samples can be selected with a single click of the mouse and run checks can also be easily performed.

New RNA Integrity Score
The updated QIAxcel ScreenGel software now features the QIAGEN RNA Integrity Score (RIS), which enables objective quality measurement of analyzed samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.

Support of 21 CFR Part 11 compliance
QIAxcel ScreenGel software includes specific features that support the technical requirements of 21 CFR Part 11 regulations. Security features such as the following are available:
Password-protected login to prevent unauthorized access and data manipulation
Audit trail documentation for configuration files and system events
Automatic saving and archiving of write-protected raw data
Secure user management

Different user profiles are available (Routine, Basic, Advanced, Admin) and are password protected (user login is required) for increased security. A simplified interface minimizes the need for user training and makes the software especially attractive for inexperienced users.

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Application Notes
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The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.
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Brochures & Guides
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For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software


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For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument
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Addressing critical factors and new solutions
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A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher
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Additional Resources
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Method file for use with BioCalculator Software
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Method file for use with QIAxcel ScreenGel Software
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Method file for use with BioCalculator Software
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Method file for use with QIAxcel ScreenGel Software
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Method file for use with BioCalculator Software
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Method file for use with QIAxcel ScreenGel Software
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Process Profile and Method file for use with QIAxcel ScreenGel Software
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FAQs
21
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Kit Handbooks
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For optimal DNA size determination
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For optimal RNA fragment size determination
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QIAxcel DNA High Resolution Kit, QIAxcel DNA Screening Kit, QIAxcel DNA Fast Analysis Kit
QIAxcel およびQIAxcel Advanced 装置を用いたDNA フラグメントの自動解析
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For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments
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For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments
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For calibration of signal intensity
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Instrument User Manuals
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QIAxcel システムで使用
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Important note regarding new declaration of conformity and instrument symbols
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For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.5
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Handbooks & Protocols
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The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.
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Operating Software
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This software may only be downloaded by registered users with a valid QIAxcel ScreenGel software license. If you do not have a valid software license, contact your QIAGEN sales representative.
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Instructions on how to install and set up QIAxcel ScreenGel software.
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