For multiplex amplification of the CODIS and ESS loci, SE33, DYS391 and Amelogenin, with an innovative Quality Sensor
  • Integrated Quality Sensor to support decision making
  • CODIS and European standard markers in one reaction
  • PCR speed of around 60 minutes for faster results
  • Reduced allelic overlap and unambiguous results
  • Robust gender typing, even with degraded or inhibited samples

The Investigator 24plex QS Kit is for human identification, allowing multiplex amplification of the CODIS (Combined DNA Index System) core loci, the ESS (European Standard Set) markers, SE33, DYS391, D2S1338, D19S433 and Amelogenin. The kit includes an innovative Quality Sensor. This Quality Sensor means that the Investigator 24plex QS Kit generates additional, valuable data for your quality control and performance checks. The kit uses QIAGEN's fast-cycling technology, which enables PCR in just 60 minutes. Highly sensitive, yielding rapid and reliable results from trace DNA, the Investigator 24plex QS Kit is suitable for all forensic applications and paternity testing. Once you've tried it, you'll always insist on Quality Sensor!

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Product Cat. no. List price:
Investigator 24plex QS Kit (100)
Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder 24plex, DNA size standard 24plex (BTO), and nuclease-free water
382415
$3,149.00
Investigator 24plex QS Kit (400)
Primer mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, allelic ladder 24plex, DNA size standard 24plex (BTO), and nuclease-free water
382417
$11,173.00
Matrix Standard BT6 (50)
Matrix standard for 6-FAM, BTG, BTY, BTR2, BTP, and BTO, for Applied Biosystems 3500 Genetic Analyzers
386224
$160.00
DNA Size Standard 24plex (BTO)
DNA Size Standard 24plex (BTO) for 800 reactions
386037
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The Investigator 24plex QS Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.
Investigator 24plex Kits are not available in all countries; please enquire.
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Performance

The Investigator 24plex QS Kit utilizes fast-cycling technology to provide reliable results in approximately 60 minutes, which is significantly faster than kits from other suppliers.

The novel Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology that has been further optimized for ease of handling and increased speed and sensitivity. Assay performance tests in the presence of increasing concentrations of inhibitors showed both high sensitivity and superior inhibitor resistance. This means reliable, high-quality results.

The Investigator 24plex QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

The recommended amount of DNA under standard conditions is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2 to 2 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator 24plex QS Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the CODIS and ESS markers, SE33, DYS391, D2S1338, D19S433 and Amelogenin. Carefully designed primers minimize the overlap of alleles of different makers, thereby minimizing the likelihood of misinterpretation of data.

The innovative Quality Sensors provides additional information on the sample quality and PCR success beyond the profile of the sample. With two Quality Sensors being amplified as a positive control, it is possible to determine:

  • If the PCR amplification worked
  • If there was any DNA in the sample
  • If the sample was degraded
  • If inhibitors in the sample prevented successful PCR

This additional information will greatly enhance how you analyze your data and aid you in making crucial decisions with respect to further sample processing. Ultimately, the Quality Sensors will help you reduce unnecessary re-runs, saving you time and money.

The optimized design of the two gender markers, Amelogenin and DYS391, enables the Investigator 24plex QS Kit to have both at a small fragment size, making the gender typing extremely robust. Even for populations with Amelogenin Null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.

Procedure

The STR markers and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator 24plex Plus Kit dyes and corresponding markers".

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof Mixture Software or Applied Biosystems GeneMapper ID-X. See the table "Technical specifications" for more information.

Table 1. Investigator 24plex QS Kit dyes and corresponding markers
Dye Marker            
6-FAM Amelogenin TH01 D3S1358 vWA D21S11    
BTG TPOX DYS391 D1S1656 D12S391 SE33    
BTY D10S1248 D22S1045 D19S433 D8S1179 D2S1338    
BTR2 D2S441 D18S51 FGA        
BTP QS1 D16S539 CSF1PO D13S317 D5S818 D7S820 QS2

 

Table 2. Investigator 24plex QS Kit technical specifications
Category Details
Volume per amplification reaction 25 μl
Matrix BT6
Fluorescence labels 6-FAM, BTG, BTY, BTR2, BTO, BTP
Genetic analyzers Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis QIAGEN Investigator IDproof Mixture Software, or Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator 24plex QS Kit is for rapid and reliable generation of DNA profiles from blood, buccal swabs and forensic stains. It is highly suited for:

  • Criminal database and forensic casework samples
  • Paternity testing
  • Population genetics studies
  • Other human identification and forensics applications

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For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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