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ipsogen BCR-ABL1 Mbcr IS-MMR Kits

For the quantification of BCR-ABL1 Mbcr b3a2 & b2a2 fusion gene transcripts and results alignment on the international scale
  • Results on the international scale (IS) and MMR reporting
  • One IS-MMR calibrator per run to prevent drifting
  • High reliability using EAC technology and single plasmid standards
  • Optimal quality control and ISO 13485 certification
  • Easy workflow with ready-to-use solutions
ipsogen BCR-ABL1 Mbcr IS-MMR Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection and quantitation of BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression.
Cat No./ID: 670713
ipsogen BCR-ABL1 Mbcr IS-MMR Kit (24)
For 24 reactions: High Positive RNA Control, IS-MMR Calibrator, Mbcr and ABL Single Plasmid Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL Mbcr Fusion Gene
Cat No./ID: 670813
ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit (24)
For 24 reactions: Reverse transcriptase, 5x RT buffer, dNTP mix, Random primers, RNase Inhibitor, DTT, High Positive RNA Control, IS-MMR Calibrator, Mbcr and ABL Single Plasmid Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL Mbcr Fusion Gene, qPCR Master mix, ROX II fluorescent dye
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
0
Single plasmid for BCR-ABL gene transcript.
1
ABL control gene transcript.
2
Accurate detection of Mbcr plasmid standards, high positive RNA control, and IS-MMR calibrator.
Mbcr plasmid standards (SP1-SP6), 101, 102, 103, 105, and 106 copies/5 μl.
3
Reliable detection of ABL plasmid standards, high positive RNA control, and IS-MMR calibrator.
ABL plasmid standards (SP3–SP6), 103, 104, 105, and 106 copies/5 μl.
Performance
To ensure highest sensitivity, ipsogen BCR-ABL1 Mbcr IS-MMR Kits have been optimized to detect low levels of BCR-ABL p210 b2a2 or b3a2 transcripts (see figures Accurate detection of Mbcr plasmid standards, high positive RNA control, and IS-MMR calibrator and Reliable detection of ABL plasmid standards, high positive RNA control, and IS-MMR calibrator). The sensitivity has a limit of detection (LOD) of 0.0069.

Principle
ipsogen BCR-ABL1 Mbcr IS-MMR Kits are ready-to-use kits for the detection of BCR-ABL p210 b2a2 or b3a2 transcripts using real-time PCR. The kits are based on the amplification and detection of specific BCR-ABL p210 b2a2 or b3a2 transcripts, relative to ABL control gene expression, in total RNA. The kits provide high levels of specificity, sensitivity, and reproducibility. Each ipsogen BCR-ABL1 Mbcr IS-MMR Kit provides 4 standard dilutions for ABL and 5 standard dilutions for Mbcr. Use of a single plasmid for BCR-ABL1 and ABL enables reproducible quantification of transcripts and limits variability. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations (see figures Single plasmid for BCR-ABL gene transcript and ABL control gene transcript). The kits also include an IS-MMR calibrator allowing conversion of results to the international scale for accurate measurement of molecular response and major molecular response (MMR) status reporting. A high positive control RNA is also included to monitor the reverse transcription and amplification steps of ABL and BCR-ABL Mbcr during transcript quantification. The ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit includes reagents for reverse transcription-PCR and real-time PCR.


Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using ipsogen BCR-ABL1 Mbcr IS-MMR Kits allows detection and quantification of BCR-ABL p210 b2a2 or b3a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications
The ipsogen BCR-ABL1 Mbcr IS-MMR Kits enable sensitive and reliable detection and quantification of BCR-ABL p210 b2a2 or b3a2 transcripts, conversion of results to the international scale, and MMR reporting, for research use only.
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