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artus Parvo B19 PCR Kits RUO

For quantitative detection of parvovirus DNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 0.2 IU/µl (ABI PRISM 7000)
  • Accurate quantitation using the 5 standards supplied
artus Parvo B19 PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for reliable parvovirus B19 DNA detection and quantitation.
Cat No./ID: 4504005
artus Parvo B19 LC PCR Kit (96) RUO
For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, Mg Solution, 5 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4504105
artus Parvo B19 TM PCR Kit (96) RUO
For 96 reactions on the ABI PRISM 7000, 7700, 7900HT SDS: Master, 5 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Performance

To ensure sensitivity, artus Parvo B19 PCR Kits have been optimized to detect low numbers of parvovirus B19 DNA. The analytical sensitivity of the artus Parvo B19 TM PCR Kit is 0.2 IU/µl in the PCR on the ABI PRISM 7000 SDS.

Performance of artus Parvo B19 PCR Kits
Kit  artus Parvo B19 LC PCR Kitartus Parvo B19 TM PCR Kit
Sample type  Serum or plasma Serum or plasma
Analytical sensitivity  125 IU/ml (plasma, QIAamp UltraSens Virus Kit) 0.2 IU/µl in the PCR

Principle

artus Parvo B19 PCR Kits are based on the amplification and simultaneous detection of a specific region of the parvovirus B19 genome using real-time PCR. The kits provide high levels of specificity and sensitivity.

Each artus Parvo B19 PCR Kit provides 5 quantitation standards (see figure "Reliable quantitation of parvovirus B19 load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical parvovirus B19 PCR is not reduced.

Specifications of artus Parvo B19 PCR Kits
Kit  artus Parvo B19 LC PCR Kitartus Parvo B19 TM PCR Kit
Sample types  Serum or plasma Serum or plasma
Amplicon  259 bp region of the parvovirus B19 genome 76 bp region of the parvovirus B19 genome

Procedure

artus Parvo B19 PCR Kits provide all necessary reagents optimized for reliable parvovirus B19 DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix (and Mg solution, LC kit only), and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For DNA purification, we recommend using the QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit.

Applications
artus Parvo B19 PCR Kits enable the rapid and sensitive detection of parvovirus B19 DNA from serum and plasma with accurate quantification. The kits are available for use on the LightCycler 1.1/1.2/1.5 Instruments or the ABI PRISM 7000, 7700, 7900HT SDS.

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