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artus Malaria RG PCR Kit RUO

For quantitative detection of Plasmodium specific DNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 0.5 copies/µl
  • Accurate quantitation using the 4 standards supplied
The artus Malaria RG PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable Plasmodium DNA detection and quantitation.
Cat No./ID: 4601203
artus Malaria RG PCR Kit (24) RUO
For 24 reactions on the Rotor-Gene 3000: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Performance

To ensure sensitivity, the artus Malaria RG PCR Kit has been optimized to detect low copy numbers of Plasmodium DNA. The analytical detection limit of the artus Malaria RG PCR Kit is 0.5 copies/μl in the PCR on the Rotor-Gene 3000.

For reliable specificity, testing of the artus Malaria RG PCR Kit was carried out using various Plasmodium species, including P. falciparum, P. vivax, P. ovale, and P. malariae, and related pathogens. 

Performance of artus Malaria RG PCR Kit
Kitartus Malaria RG PCR Kit
Sample type EDTA blood
Analytical sensitivity 0.5 copies/µl in the PCR
 Specificity  Plasmodium species, including P. falciparum, P. vivax, P. ovale, and P. malariae

Principle

The artus Malaria RG PCR Kit is based on the amplification and simultaneous detection of a specific region of the Plasmodium genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus Malaria RG PCR Kit provides 4 quantitation standards (see figure "Reliable quantitation of Plasmodium load"). Use of the standards enables accurate quantitation of pathogen load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical Plasmodium PCR is not reduced.

Specifications of artus Malaria RG PCR Kit
Kitartus Malaria RG PCR Kit
Sample type EDTA blood
Amplicon 163 bp region of the Plasmodium genome

Procedure
The artus Malaria RG PCR Kit provides all necessary reagents optimized for reliable Plasmodium DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
Applications
The artus Malaria RG PCR Kit enables the rapid and sensitive detection of DNA of the Plasmodium species P. falciparum, P. vivax, P. ovale, and P. malariae from EDTA blood with accurate quantification. The kit is available for use on the Rotor-Gene 3000 instrument.
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