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artus C. trachomatis Plus PCR Kits CE

For detection of Chlamydia trachomatis DNA using real-time PCR
  • Compliance with EU IVD Directive 98/79/EC
  • High reliability using the internal control
  • Highly sensitive detection of as few as 300 be/ml (RG kit)
  • Excellent specificity using 1 target gene for detection
artus C. trachomatis Plus PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for reliable C. trachomatis DNA detection for in vitro diagnostic use. The detection of 1 target provides a highly sensitive and specific detection of known C. trachomatis strains. The artus C. trachomatis Plus kits do not detect cryptic-plasmid free variants of C. trachomatis. For details, see Important Note.
Cat No./ID: 4559263
artus C. trachomatis Plus RG PCR Kit (24) CE
For 24 reactions on Rotor-Gene Q instruments: Master, Mg Solution, Positive Control, Negative Control, Water (PCR grade)
Cat No./ID: 4559265
artus C. trachomatis Plus RG PCR Kit (96) CE
For 96 reactions on Rotor-Gene Q instruments: Master, Mg Solution, Positive Control, Negative Control, Water (PCR grade)

artus C. trachomatis Plus PCR Kits CE are intended for in vitro diagnostic use in Europe.


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Reliable detection of the positive control for C. trachomatis.
Detection of the positive control (C. trachomatis Plus LC/RG Positive Control) on the Rotor-Gene using the artus C. trachomatis Plus RG PCR Kit. NTC: no template control.
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C. trachomatis.
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CE-IVD-marked.
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In vitro diagnostic medical device.
Performance

To ensure highest sensitivity, artus C. trachomatis Plus PCR Kits have been optimized to detect low numbers of C. trachomatis DNA. The analytical sensitivity of the artus C. trachomatis Plus RG PCR Kit is 300 bacteria genome equivalents/ml (be/ml) in consideration of the purification from swabs using the QIAamp DNA Mini Kit.

Performance characteristics of the artus C. trachomatis Plus RG PCR Kit
Kitartus C. trachomatis Plus RG PCR Kit
Validated sample types Urine, swabs, or semen
Analytical sensitivity 300 be/ml (swabs, QIAamp DNA Mini Kit)
 Specificity  C. trachomatis, including serovars A–K, Ba, and LGV I–III

Principle

artus C. trachomatis Plus PCR Kits are based on the amplification and simultaneous detection of one target gene of C. trachomatis, the 111 bp region of the cryptic plasmid, using real-time PCR. The detection of one target guarantees a highly sensitive and specific detection of known C. trachomatis strains. The artus C. trachomatis Plus PCR Kits do not detect cryptic-plasmid free variants of C. trachomatis.The kits provide high levels of specificity, sensitivity, and reproducibility.

The kits provide positive and negative controls (see figure "Reliable detection of the positive control for C. trachomatis". In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR.

Specifications of artus C. trachomatis Plus RG PCR Kit
Kitartus C. trachomatis Plus RG PCR Kit
Validated sample types Urine, swabs, or semen
Amplicons 111 bp region of the cryptic plasmid

Procedure

artus C. trachomatis Plus PCR Kits provide all necessary reagents optimized for reliable C. trachomatis DNA detection. Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For DNA purification, the QIAamp Viral RNA Mini Kit or QIAamp DNA Mini Kit is to be used to ensure legally validated performance.

Applications
artus C. trachomatis Plus PCR Kits allow the rapid and sensitive detection of C. trachomatis DNA from urine, swabs, and semen. Kits are available for use on LightCycler 1.1/1.2/1.5/2.0 Instruments or Rotor-Gene Q instruments.
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