artus Borrelia LC PCR Kit CE
For quantitative detection of Borrelia DNA using real-time PCR
The artus Borrelia LC PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable detection and quantitation of DNA of all members of Borrelia burgdorferi sensu lato including Borrelia species that are pathogenic for humans (B. burgdorferi sensu stricto, B. garinii, and B. afzelii). The kit is intended for in vitro diagnostic use.
The artus Borrelia LC PCR Kit CE is intended for in vitro diagnostic use in Europe.
To ensure highest sensitivity, the artus Borrelia LC PCR Kit has been optimized to detect low copy numbers of Borrelia DNA. The analytical sensitivity of the kit is 3.3 copies/μl in the PCR on the LightCycler 1.1/1.2/1.5 Instrument and 2.8 copies/μl in the PCR on the LightCycler 2.0 Instrument.
For highest specificity, validation of the artus Borrelia LC PCR Kit was carried out using various Borrelia species, including B. burgdorferi, B. garinii, B. afzelii, B. valaisiana, B. hermsii, and related pathogens.
The artus Borrelia LC PCR Kit is based on the amplification and simultaneous detection of a specific region of the Borrelia genome using real-time PCR. The kit provides high levels of specificity, sensitivity, and reproducibility.
Each artus Borrelia LC PCR Kit provides 4 Borrelia quantitation standards (see figure "Reliable quantitation of Borrelia load"). Use of the standards enables accurate quantitation of pathogen load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical Borrelia PCR is not reduced.
The artus Borrelia LC PCR Kit provides all necessary reagents optimized for reliable Borrelia DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
For DNA purification, the QIAamp DNA Mini Kit is to be used.
The artus Borrelia LC PCR Kit enables rapid and sensitive detection of Borrelia DNA with highly accurate quantification. The kit has been tested for use with DNA purified from skin biopsies, synovial fluid, CSF, blood, ticks, and culture using the QIAamp DNA Mini Kit and is validated for use with DNA purified from CSF using the QIAamp DNA Mini Kit. The kit is available for use on the LightCycler 1.1/1.2/1.5/2.0 Instruments.
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