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artus Aspergillus diff. RG PCR Kit RUO

For amplification and differentiation of Aspergillus spp. DNA
  • High reliability using the internal control
  • Sensitive detection of Aspergillus spp.
  • Differentiation of Aspergillus fumigatus/terreus/flavus DNA
  • Ready-to-use reaction mixes
The artus Aspergillus diff. RG PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit includes a reaction mix containing all reagents and enzymes for amplification, detection, and differentiation of Aspergillus fumigatus/terreus/flavus DNA in whole blood.
Cat No./ID: 4640203
artus Aspergillus diff. RG PCR Kit (24) RUO
For 24 reactions: Master, Magnesium Solution, Positive Control, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Performance
The analytical sensitivity (without sample preparation) of the artus Aspergillus diff. RG PCR Kit was determined by probit analysis with a probability of 95% for positive samples. Analytical sensitivity for all 3 targets was less than or equal to 10 copies/µl.
Performance of artus Aspergillus diff. RG PCR Kit
Kit artus Aspergillus diff. RG PCR Kit
Sample type Whole blood
Analytical sensitivity Less than or equal to 10 copies/µl
 Specificity  Tested with Aspergillus fumigatus/terreus/flavus DNA; no cross-reactivity with Aspergillus niger/ustus/versicolor DNA

Principle
The artus Aspergillus RG PCR Kit is based on the amplification and simultaneous detection of a specific region of the Aspergillus fumigatus/terreus/flavus genomes using real-time PCR. The kit provides high levels of specificity and sensitivity.

The kit provides a positive control for A. fumigatus/terreus/flavus DNA. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCRs.
 
Specifications of artus Aspergillus diff. RG PCR Kit
Kit artus Aspergillus diff. RG PCR Kit
Sample type Whole blood
Amplicon 110 bp regions of the A. fumigatus/terreus/flavus genomes

Procedure
The artus Aspergillus diff. RG PCR Kit provides all necessary reagents optimized for reliable Aspergillus fumigatus/terreus/flavus DNA detection and differentiation. Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook. For DNA purification, we recommend using the QIAamp UCP PurePathogen Blood Kit.

Applications
The artus Aspergillus diff. RG PCR Kit enables rapid and sensitive detection of Aspergillus spp. DNA from whole blood. The kit enables discrimination of A. fumigatus/terreus/flavus DNA using specific probes.

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