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Investigator Triplex AFS QS Kit

For multiplex amplification of FGA (FIBRA), SE33 (ACTBP2), and Amelogenin

  • Simultaneous amplification of 3 markers
  • Includes an internal control for PCR efficiency
  • Highly suited for low-copy and degraded DNA samples
  • Highly sensitive and inhibitor resistant

The Investigator Triplex AFS QS Kit is for forensic stain pre-selection, which serves as a starting point for genotyping strategies. The genetic analysis is calibrated with DS-30 matrix.

Cat No./ID: 380317
Investigator Triplex AFS QS Kit (400)
$7,152.00
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Primer Mix including an Internal Control (QS), Reaction Mix, Control DNA, DNA Size Standard 550 (ROX), Allelic Ladder Triplex AFS QS, and Nuclease-Free Water

The Investigator Triplex AFS QS Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.



Performance

The Investigator Triplex AFS QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers.

The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.1–1.0 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator Triplex AFS QS Kit enables the simultaneous amplification of the two polymorphic STR loci FGA (FIBRA) and SE33 (ACTBP2), and the gender-specific Amelogenin. The kit includes optimized primers and reagents, and the relevant DNA control and allelic ladder.

The QS in the name stands for Quality Sensor. This special feature is an internal PCR control that provides information on the presence of PCR inhibitors and the efficiency of the PCR.

Procedure

FGA (FIBRA), SE33 (ACTBP2), and Amelogenin are simultaneously amplified in a single PCR and labeled with 6-FAM. The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.

Technical specifications
Volume per PCR reaction 25 μl
Matrix DS-30
Fluorescence labels 6-FAM, ROX
Genetic analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis QIAGEN Investigator IDproof or Investigator IDproof Mixture Software, or Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator Triplex AFS QS Kit is preferentially used for forensic prescreening strategies or for low-copy and degraded DNA samples that do not give distinct results with complex multiplexes. It enables rapid and reliable DNA genotyping of blood, buccal swabs, and forensic stains.

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For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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