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Investigator Argus X-12 Kit

For multiplex amplification of twelve X-chromosomal loci plus Amelogenin

  • Unique kit for analyzing X-chromosomal markers
  • Complements the Investigator Argus Y-12 QS Kit
  • Heightened discriminatory power for deficiency cases
  • No overlapping allelic ranges within a panel

The Investigator Argus X-12 Kit is a unique tool for analyzing X-chromosomal markers, which are highly informative for kinship and paternity testing, and for gonosomal STR analysis of forensic stains. This kit complements the Investigator Argus Y-12 QS Kit, especially in complicated deficiency cases. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.

Cat No./ID: 383215
Investigator Argus X-12 Kit (100)
$4,081.00
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Primer Mix, Reaction Mix, Control DNA, Multi Taq2 Polymerase, DNA Size Standard 550 (BTO), Allelic Ladder Argus X-12, and Nuclease-Free Water

The Investigator Argus X-12 Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.



Performance

The Investigator Argus X-12 Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers.

The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–0.5 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator Argus X-12 Kit enables simultaneous amplification of 12 X-chromosomal STR loci (see table "X-chromosomal markers amplified with the Investigator Argus X-12 Kit"), which are highly informative for kinship and paternity testing, as well as population genetics and anthropological studies. The heightened discriminatory power of the kit meets the demands of complicated deficiency cases involving at least one female. In addition, this kit is highly suited for analysis of forensic stains, such as female traces on a male background, and it is an excellent complement to the Investigator Argus Y-12 QS Kit. The markers of the Investigator Argus X-12 are clustered into 4 linkage groups (3 markers per group, see table "X-chromosomal markers amplified with the Investigator Argus X-12 Kit"), and thus each set of 3 markers is handled as a haplotype for genotyping.

X-chromosomal markers amplified with the Investigator Argus X-12 Kit
Linkage groupMarkers
1 (Xp22) DXS8378 – DXS10135 – DXS10148
2 (Xq11) DXS7132 – DXS10074 – DXS10079
3 (Xq26) HPRTB – DXS10101 – DXS10103
4 (Xq28) DXS7423 – DXS10134 – DXS10146
Procedure

The 12 polymorphic X-chromosomal loci and Amelogenin are simultaneously amplified in a single PCR and labeled with the dyes listed in the table "Investigator Argus X-12 Kit dyes and corresponding markers". 

The amplified loci undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.

Investigator Argus X-12 Kit dyes and corresponding markers
DyeMarkers 
6-FAM Amelogenin  DXS10103  DXS8378 DXS7132 DXS10134
BTG DXS10074  DXS10101  DXS10135
BTY DXS7423 DXS10146 DXS10079
BTR HPRTB DXS10148
Technical specifications
Volume per amplification reaction 25 μl
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis QIAGEN Investigator IDproof or Investigator IDproof Mixture Software, or Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator Argus X-12 Kit enables simultaneous amplification of 12 X-chromosomal markers, and is highly suitable for:

Kinship and paternity testing, especially deficiency cases
Population genetics and anthropological studies
Analysis of stains with female traces on a male background

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