For multiplex amplification of twelve X-chromosomal loci plus Amelogenin
Unique kit for analyzing X-chromosomal markers
Complements the Investigator Argus Y-12 QS Kit
Heightened discriminatory power for deficiency cases
No overlapping allelic ranges within a panel
The Investigator Argus X-12 Kit is a unique tool for analyzing X-chromosomal markers, which are highly informative for kinship and paternity testing, and for gonosomal STR analysis of forensic stains. This kit complements the Investigator Argus Y-12 QS Kit, especially in complicated deficiency cases. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.
Primer Mix, Reaction Mix, Control DNA, Multi Taq2 Polymerase, DNA Size Standard 550 (BTO), Allelic Ladder Argus X-12, and Nuclease-Free Water
The Investigator Argus X-12 Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.
The Investigator Argus X-12 Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers.
The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–0.5 ng DNA, and reliable results with <0.1 ng DNA.
The Investigator Argus X-12 Kit enables simultaneous amplification of 12 X-chromosomal STR loci (see table "X-chromosomal markers amplified with the Investigator Argus X-12 Kit"), which are highly informative for kinship and paternity testing, as well as population genetics and anthropological studies. The heightened discriminatory power of the kit meets the demands of complicated deficiency cases involving at least one female. In addition, this kit is highly suited for analysis of forensic stains, such as female traces on a male background, and it is an excellent complement to the Investigator Argus Y-12 QS Kit. The markers of the Investigator Argus X-12 are clustered into 4 linkage groups (3 markers per group, see table "X-chromosomal markers amplified with the Investigator Argus X-12 Kit"), and thus each set of 3 markers is handled as a haplotype for genotyping.
X-chromosomal markers amplified with the Investigator Argus X-12 Kit
DXS8378 – DXS10135 – DXS10148
DXS7132 – DXS10074 – DXS10079
HPRTB – DXS10101 – DXS10103
DXS7423 – DXS10134 – DXS10146
The 12 polymorphic X-chromosomal loci and Amelogenin are simultaneously amplified in a single PCR and labeled with the dyes listed in the table "Investigator Argus X-12 Kit dyes and corresponding markers".
The amplified loci undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.
Investigator Argus X-12 Kit dyes and corresponding markers
Volume per amplification reaction
6-FAM, BTG, BTY, BTR, BTO
ABI PRISM 310, ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis
QIAGEN Investigator IDproof or Investigator IDproof Mixture Software, or Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
The Investigator Argus X-12 Kit enables simultaneous amplification of 12 X-chromosomal markers, and is highly suitable for:
Kinship and paternity testing, especially deficiency cases
Population genetics and anthropological studies
Analysis of stains with female traces on a male background
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)