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mericon RR Soy Kit

For real-time PCR detection of Roundup Ready soy in food or animal feed

  • Highly sensitive and specific detection of Roundup Ready soy
  • Streamlined detection protocol
  • Dedicated mericon DNA isolation kit available
  • PCR run time of 82 minutes on the QIAGEN Rotor-Gene Q
  • Full license for PCR without additional costs
The mericon RR Soy Kit is designed for the target-specific detection of Roundup Ready soy in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for pathogen detection and ingredient authentication. The mericon RR Soy Kit performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
Cat No./ID: 291113
mericon RR Soy Kit (24)
$562.00
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For 24 reactions: PCR Assay RR Soy, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution
The mericon RR Soy Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The mericon RR Soy Kit is designed for the detection of Roundup Ready soy in food and animal feed. The mericon RR Soy Kit can detect as little as 10 copies of target DNA in a reaction and exhibits high specificity for Roundup Ready soy . No cross-reactivity was observed with other plant or animal species, GMOs or genetic constructs not found in Roundup Ready soy using 50 ng of tested DNA (see the table "Results of mericon RR Soy Kit cross-reactivity study").

Results of mericon RR Soy Kit cross-reactivity study

 Species, GMO, or
 genetic construct
 Result  Species, GMO, or
 genetic construct
 Result
 Roundup Ready soy  +  Bt11 corn  –
 Bt176 corn  –  TC1507 corn  –
 StarLink corn  –  T25 corn  –
 MON810 corn  –  NK603 corn  –
 GA21 corn  –  Rice  –
 Potato  –  Rye  –
 Pig  –  Barley  –
 Wheat  –  Sheep  –
Principle

The mericon Roundup Ready Soy Kit is designed for the qualitative detection of the genetically modified organism (GMO) Roundup Ready soy in food and animal feed samples. The genetic modification in the RR soy line provides resistance to the active compound glyphosate in herbicides, which is detected by the mericon RR Soy Kit. RR soy is the most frequently used genetically modified soy line.

Detection of GMOs using real-time PCR is based on the amplification of a specific region of the relevant GMO genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets either a genetic construct that is commonly used in GMOs or a DNA sequence that is specific for a particular GMO. These targets were verified bioinformatically and experimentally. 

Summary of possible PCR outcomes
Internal control amplifiedTested DNA amplified Meaning
            +            +  Sample is positive for the target DNA
            +            –  Sample is negative for the target DNA
            –            –  PCR failed due to inhibition
Procedure

DNA is extracted from a sample of food or animal feed using a dedicated sample preparation kit. QIAGEN's DNeasy mericon Food Kit is suitable for a wide range of food matrices, including those with high inhibitor contents.

The isolated, pure DNA is assayed using a streamlined real-time PCR protocol that returns results in 82 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications
The mericon RR Soy Kit is for real-time PCR-based detection of Roundup Ready soy in food, animal feed, and pharmaceutical products.
Features
Specifications
Quantitative/qualitative Qualitative
Recommended sample prep DNeasy mericon Food Kit or DNeasy Plant Kits
RUO/CE/ASR RUO
Sample type Food
Thermal cycler Rotor-Gene Q and other cyclers
What detected GMO DNA
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