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mericon MON 810 Corn Kit

For real-time PCR detection of MON 810 corn in food or animal feed

  • Highly sensitive and specific detection of GMOs containing MON 810 corn
  • Streamlined detection protocol
  • Dedicated mericon DNA isolation kit available
  • PCR run time of 82 minutes on the QIAGEN Rotor-Gene Q
  • Full license for PCR without additional costs
The mericon MON 810 Corn Kit is designed for the target-specific detection of MON 810 corn in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for pathogen detection and ingredient authentication. The mericon MON 810 Corn Kit performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
Cat No./ID: 291073
mericon MON 810 Corn Kit (24)
$562.00
Add To Cart
For 24 reactions: PCR Assay MON 810 Corn, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution
Cat No./ID: 291075
mericon MON 810 Corn Kit (96)
$2,019.00
Add To Cart
For 96 reactions: PCR Assay MON 810 Corn, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution
The mericon MON 810 Corn Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The mericon MON 810 Corn Kit is designed for the detection of MON 810 corn in food and animal feed. The mericon MON 810 Corn Kit can detect as little as 10 copies of target DNA in a reaction and exhibits high specificity for MON 810 corn. No cross-reactivity was observed with other genetically modifiied organisms or with other plant or animal species  using 50 ng of tested DNA (see the table "Results of mericon MON 810 Corn Kit cross-reactivity study").

Results of mericon MON 810 Corn Kit cross-reactivity study

 Species, GMO, or
 genetic construct
 Result  Species, GMO or 
 genetic construct
 Result
 MON810 corn  +  EH92-527 potato  –
 Bt176 corn  –  H7-1 sugar beet  –
 Bt11 corn  –  Soy  –
 MIR604 corn  –  Barley  –
 3272 corn  –  Potato  –
 NK603 corn  –  Rice  –
 MON863 corn  –  Wheat  –
 TC1507 corn  –  Rye  –
 59122 corn  –  Cattle  –
 98140 corn  –  Pig  –
 Roundup Ready soy  –  Sheep  –
 305423 soy  –  Goat  –
 356043 soy  –

Principle

The mericon MON 810 Corn Kit was developed for the qualitative detection of the genetically modified organism (GMO) MON 810 corn (one of the most frequently used genetically modified corn lines worldwide) in food and animal feed samples. The corn line MON 810 was developed through a genetic modification resulting in resistance against the European corn borer (Ostrinia nubilalis) by producing Bt‑Toxins of Bacillus thuringiensis.

Detection of GMOs using real-time PCR is based on the amplification of a specific region of the relevant GMO genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets either a genetic construct that is commonly used in GMOs or a DNA sequence that is specific for a particular GMO. These targets were verified bioinformatically and experimentally. 

Summary of possible PCR outcomes
Internal control amplifiedTested DNA amplified Meaning
            +            +  Sample is positive for the target DNA
            +            –  Sample is negative for the target DNA
            –            –  PCR failed due to inhibition
Procedure

DNA is extracted from a sample of food or animal feed using a dedicated sample preparation kit. QIAGEN's DNeasy mericon Food Kit is suitable for a wide range of food matrices, including those with high inhibitor contents.

The isolated, pure DNA is assayed using a streamlined real-time PCR protocol that returns results in 82 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications
The mericon MON 810 Corn Kit is for real-time PCR-based detection of GMOs containing MON 810 corn in food, animal feed, and pharmaceutical products.
Features
Specifications
Quantitative/qualitative Qualitative
Recommended sample prep DNeasy mericon Food Kit or DNeasy Plant Kits
RUO/CE/ASR RUO
Sample type Food
Thermal cycler Rotor-Gene Q and other cyclers
What detected GMO DNA
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