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BLU-V System

For differentiation of live and dead cells

  • Simultaneous photo-activation of up to 12 samples
  • Constant and uniform light dosage for optimal PMA activation
  • No heating of samples during PMA photo-activation



The BLU-V System allows the neutralization of the DNA of dead organisms through the use of photo-activation on intercalating agents. Neutralizing the DNA of dead organisms allows the downstream detection of DNA of living cells by conventional PCR or real-time PCR.

The BLU-V System is a thermally stable instrument that generates a constant and uniform light dosage to allow simultaneous photo-activation of 12 Propidium Monoazide (PMA) treated samples, permitting differentiation of live and dead microorganisms.
Cat No./ID: 9002300
BLU-V System
Instrument for photoactivation of dyes, includes one
BLU-V Sample Holder
Cat No./ID: 9022908
BLU-V Incubation Box
Box for up to 12 tubes (tube volumes up to 2 ml) for light protection during incubation
Cat No./ID: 9022909
BLU-V Sample Holder
Sample holder for up to 12 tubes (tube volumes up to 2 ml)
BLU-V System are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Principle
The BLU-V System allows the neutralization of the DNA of dead organisms through the use of photo-activation on intercalating agents. Neutralizing the DNA of dead organisms allows the downstream detection of DNA of living cells by conventional PCR or real-time PCR.
Procedure
Bacterial species are prepared and treated with PMA. Following activation of the compound by illumination with a specific wavelength in the BLU-V System, the reagent is irreversibly bound to DNA of dead cells, preventing PCR amplification. The BLU-V System is thermally stable with a constant and uniform light dose, guaranteeing the maximum efficiency in the binding of the reagent to DNA. Efficient suppression of amplification of such modified DNA allows preferential detection of live cells.
Applications
Live/dead differentiation can play an important role in procedures such as — hygiene testing to measure the success of decontamination processes, water testing, to distinguish between live and dead Legionella for regulatory compliance, and to monitor the efficiency of pathogen killing.
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