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virotype PEDV/TGEV RT-PCR Kit

The virotype PEDV/TGEV RT-PCR Kit is a highly sensitive and specific solution for simultaneous detection of RNAs from porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) in feces, tissue, oral fluid and swab samples from swine. Due to the high sensitivity of the test, pools of up to 5 individual samples can be used.
The virotype PEDV/TGEV RT-PCR Kit contains all of the reagents for real-time RT-PCR in a single ready-to-use reaction mix. With this kit, both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination. Additionally, real-time PCR identification can be conveniently carried out on the Rotor-Gene Q thermal cycler.
Cat No./ID: 283605
virotype PEDV/TGEV RT-PCR Kit (96)
For 96 reactions: Master Mix, Positive Control, Negative Control
For research use only. USA/Canada: not for use in diagnosis of disease in animals.
Principle
This multiplex real-time RT-PCR kit simultaneously detects RNAs from PEDV and TGEV. RT-PCR is based on the amplification of specific regions of the pathogen genome. It is a sensitive and specific tool for pathogen detection.

Real-time RT-PCR involves the reverse transcription of viral RNA and subsequent amplification of the cDNA by PCR. The amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterwards.

The kit uses three specific primer/probe combinations: PEDV RNA, TGEV RNA and a heterologous RNA control, detected using FAM, Quasar 670/Cy5 and HEX fluorescence, respectively. The internal control excludes the possibility of false-negative results, and the positive control verifies the functionality of the reaction mix for the amplification of the PEDV/TGEV RNA targets.
Procedure
The kit contains a ready-to-use mix with all of the necessary reagents for the detection of PEDV and TGEV RNAs and the required positive and negative controls. With this kit, both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination. RNA is extracted from the sample material (feces, tissue, oral fluid and swabs) using a suitable sample preparation kit. The extracted RNA is used for the RT-PCR, which is run on a thermal cycler.
Applications
The virotype PEDV/TGEV RT-PCR Kit is for the simultaneous detection of RNAs from porcine epidemic diarrhea virus and transmissible gastroenteritis virus in feces, tissue, oral fluid and swab samples from swine.

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