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pigtype Salmonella Ab

For detection of antibodies to Salmonella spp. in samples from swine

  • Highly sensitive and specific indirect ELISA for salmonella
  • Suitable for serum, plasma, and meat juice samples
  • User-friendly test protocol with ready-to-use colored reagents
  • Results in less than 2 hours with optional overnight protocol
pigtype Salmonella Ab is a sensitive and specific ELISA for detecting antibodies to Salmonella spp. in serum, plasma and meat juice samples from swine.
Cat No./ID: 273001
pigtype Salmonella Ab (1)
For 96 reactions: 1 Test plate (strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution
Cat No./ID: 273003
pigtype Salmonella Ab (5)
For 480 reactions: 5 Test plates (strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution
Cat No./ID: 273005
pigtype Salmonella Ab (20)
For 1920 reactions: 20 Test plates, Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution

pigtype Salmonella Ab is for veterinary use only.

In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: BFAV-B 380



Performance

pigtype Salmonella Ab is specific for the detection of antibodies to Salmonella spp. Tests were run using the pigtype Salmonella Ab Kit in the 2011 QS* ring trial. Ten serum and 10 meat juice samples were tested. All positive samples were correctly scored as being positive for salmonella antibodies. All negative samples were correctly scored as being negative for salmonella antibodies (see the table "QS ring trial results").


QS ring trial results
Sample Expected result  OD % pigtype Salmonella Ab Kit result
Serum 1 Negative   1.3  Negative
Serum 2 Positive  63.9  Positive 
Serum 3 Positive  77.9  Positive 
Serum 4 Positive  64.9  Positive 
Serum 5 Negative   4.4  Negative 
Serum 6 Negative   5.4  Negative 
Serum 7 Positive  73.2  Positive 
Serum 8 Positive   93.2  Positive 
Serum 9 Negative   5.4  Negative 
Serum 10 Positive  74.2  Positive 
Meat juice 1 Negative  3.7  Negative 
Meat juice 2 Negative   19.2  Negative 
Meat juice 3 Negative   11.2  Negative 
Meat juice 4  Negative   6.7  Negative 
Meat juice 5  Positive  44.7  Positive 
Meat juice 6  Positive  101.4  Positive 
Meat juice 7  Negative   18.9  Negative 
Meat juice 8  Positive  45.8  Positive 
Meat juice 9  Negative   23.7  Negative
Meat juice 10  Negative   17.2  Negative 
Principle

 

pigtype Salmonella Ab is an ELISA (enzyme-linked immunosorbent assay) with ready-to-use colored reagents. The test plate is coated with inactivated salmonella antigen. During the sample incubation, salmonella-specific antibodies bind to the immobilized antigen. The anti-IgG-HRP conjugate detects antibodies bound to the antigen. The color reaction is started by adding the substrate solution and stopped after 10 minutes. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of anti-Salmonella antibodies in the sample.

 

Procedure

Serum, plasma, and meat juice samples can be tested after dilution.

Samples are incubated for one hour with the test plate and if antibodies to Salmonella spp. are present, they will bind to the antigen on the plates. Unbound material is removed by rinsing.

The anti-IgG-HRP conjugate is added. It contains an HRP-labeled, anti-IgG-specific monoclonal antibody. Unbound conjugate is rinsed out.

The color reaction is started by adding the TMB Substrate Solution and stopped after 10 minutes. The optical density (OD) is measured using a spectrophotometer. The results are easy to interpret.

Applications

pigtype Salmonella Ab is for the detection of antibodies to Salmonella spp. in serum, plasma, and meat juice samples from swine.


*Qualität und Sicherheit GmbH

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