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pigtype PRRSV Ab and pigtype PRRSV Ab OF

For detection of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV)
  • Highly specific and sensitive detection of antibodies to PRRSV in porcine samples
  • Detection of antibodies to PRRSV NA and EU strains
  • Colored, ready-to-use reagents
  • Easy-to-use, short-step protocol for testing serum samples within 1.5 hours and oral fluid samples within 3 hours


pigtype
PRRSV Ab is a highly specific and sensitive ELISA for detection of antibodies to PRRSV (EU and NA virus types) in serum and plasma samples from pigs. For reliable testing of oral fluid samples from pigs we provide the pigtype PRRSV Ab OF. Both kits are effective and suitable diagnostic tools to monitor the infection and vaccination status of pig herds over extended time periods and for controlling the serological status of purchased animals.
Cat No./ID: 272753
pigtype PRRSV Ab (5)
For 480 reactions: 5 Test Plates (strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution
Cat No./ID: 272771
pigtype PRRSV Ab OF (1)
For 96 reaction: 1 Test Plate (strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution
Assays for veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area.
Principle
PRRSV is an RNA virus that is a member of the family Arteriviridae, order Nidovirales. PRRS viruses are classified into the European (EU/I) and the North American (NA/II) genotypes. PRRS virus infections can cause respiratory disease in piglets and reproductive failure in pregnant sows.

The pigtype PRRSV Ab and the pigtype PRRSV Ab OF are indirect ELISAs for detection of these viruses. The microtiter test plate is coated with recombinant PRRSV antigens, specific for NA and EU virus types. During sample incubation, antibodies specific for PRRSV bind to the immobilized antigen. Unbound material is removed by rinsing. Antibodies bound to the antigen are detected by a horseradish peroxidase (HRP) conjugate. Unbound conjugate is removed by rinsing. A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. If antibodies specific for PRRSV are present in the sample, a blue color develops, which turns yellow after the addition of Stop Solution. The optical density (OD) is measured in a spectrophotometer at 450 nm. Measurement at a reference wavelength (620–650 nm) is optional. The OD value correlates to the concentration of antibodies specific for the NA/EU strains of PRRSV in the sample.
Applications
The pigtype PRRSV Ab and pigtype PRRSV Ab OF are highly sensitive and specific solutions for detection of antibodies to PRRSV (NA and EU virus types) in serum/plasma and oral fluid samples from pigs, respectively.

For further information and validation data on QIAGEN assays, contact QIAGEN Technical Service.
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