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pigtype CSFV Erns Ab

For detection of antibodies to the Erns protein of the classical swine fever virus (CSFV)
  • Early detection of CSFV infection independently from CSFV genotype
  • Erns based antigen allows for differentiation of infected and vaccinated animals (DIVA potential)
  • Colored, ready-to-use reagents
  • Easy-to-use, short step-by-step protocol for test results in under 2.5 hours


pigtype
CSFV Erns Ab is a standardized and validated ELISA kit for reliable screening of antibodies to the Erns protein of the classical swine fever virus (CSFV) in porcine (domestic pigs, wild boar) serum and plasma samples.

Cat No./ID: 272301
pigtype CSFV Erns Ab (1)
For 96 reactions: 1 Test Plate (Strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution
Cat No./ID: 272303
pigtype CSFV Erns Ab (5)
For 480 reactions: 5 Test Plates (Strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution
Assay for veterinary use only. Regulatory requirements vary by country; product may not be available in your geographic area.
Principle
The pigtype CSFV Erns Ab is a double-antigen ELISA. The microtiter plate is coated with recombinant CSFV Erns antigen. During sample incubation, antibodies to the CSFV Erns protein bind to the immobilized antigen. Unbound material is removed by rinsing. Antibodies bound to the antigen are detected by a horseradish peroxidase (HRP) conjugate. Unbound conjugate is removed by rinsing. A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. If antibodies to the CSFV Erns protein are present in the sample, a blue color develops, which turns yellow after the addition of Stop Solution. The optical density (OD) is measured in a spectrophotometer at 450 nm. Measurement at a reference wavelength (620-650 nm) is optional. The OD value correlates to the concentration of the antibodies to the CSFV Erns protein in the sample.
Applications
pigtype CSFV Erns Ab is for detection of antibodies to the Erns protein of the classical swine fever virus (CSFV) in porcine serum and plasma samples.

The Kit is suitable as a CSFV screening test. DIVA potential: The pigtype CSFV Erns Ab enables differentiation between infected and appropriate CSFV marker-vaccinated animals (e.g., Suvaxyn® CSFV marker).

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