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flocktype Mycoplasma Ms Ab

For detection of antibodies to Mycoplasma synoviae in samples from poultry

  • Highly sensitive and specific indirect ELISA for Mycoplasma synoviae
  • Suitable for serum and plasma samples
  • User-friendly test protocol with ready-to-use reagents
  • Results in less than 1.5 hours



flocktype
Mycoplasma Ms Ab is a sensitive and specific ELISA for detecting antibodies to Mycoplasma synoviae in serum and plasma samples from chickens and turkeys.

flocktype Mycoplasma Ms Ab is for veterinary use only.

In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: FLI-B 432
Cat No./ID: 274602
flocktype Mycoplasma Ms Ab (2)
For 192 reactions: 2 Test plates (strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Anti-IgY-HRP Conjugate, TMB Substrate Solution, Stop Solution

flocktype Mycoplasma Ms Ab is for veterinary use only.

In Germany: Registered in accordance with ยง17c of the German Law on Animal Diseases. Registration No.: FLI-B 432



Performance
flocktype Mycoplasma Ms Ab is specific for the detection of antibodies to Mycoplasma synoviae. Tests were run using the flocktype Ms Ab Kit in the Deventer International Proficiency Testing Scheme (PTS) ring trial. Sample sets were from either vaccinated chickens (samples 3 and 6) or from infected chickens (samples 1, 2, 4, 7 and 8). Four positive serum samples were correctly scored as being positive for Ms antibodies (samples 1, 4, 5, and 7). Samples 3, 6, and 8 [Mg- or Mycoplasma meleagridis (Mm)-positive] correctly scored as negative. Sample 2 is an Mg-positive serum that gave a doubtful result with flocktype Mycoplasma Ms Ab (see the table Deventer ring trial Mycoplasma Ms 2012 results).


Deventer ring trial Mycoplasma Ms 2012 results
Sample  Titre  Expected result  flocktype Mycoplasma Ms Ab Kit result   Origin
 1 15433 Positive Positive   Ms ATCC+ field 35 d.p.i. 
 2 1068  Positive  Doubtful Mg F-strain 24 d.p.i. 
 3 119  Negative Negative   Poulvac Mg 21 d.p.v. 
 4 3052 Positive Positive  Ms field strain 7 d.p.i.
 5 3937 Positive  Positive  Mg/Ms  
 6 2 Negative Negative  Mg S6/85 21 d.p.v.
 7 20013  Positive Positive  Ms field strain 28 d.p.i.
 8 Negative Negative  Mm field strain 25 d.p.i.

The kit correctly scored 7 of the 8 samples.
Principle

Mycoplasma synoviae is found worldwide and causes chronic respiratory diseases in birds. Systemic infection leads to infectious synovitis. The severity of infection depends on secondary infections.

Mycoplasmas adhere to the respiratory epithelium and to erythrocytes. The cellular immune response is central to infections with M. synoviae, although the production of specific antibodies may also be induced by infections and vaccinations. For confirmation of the flocktype Mycoplasma Mg Ab results, we recommend the identification of the pathogen from tracheal swabs using PCR (e.g., with the bactotype Mycoplasma Mg/Ms PCR Kit).

flocktype Mycoplasma Ms Ab is an ELISA (enzyme-linked immunosorbent assay) with ready-to-use colored reagents. The test plate is coated with a recombinant membrane protein that is specific for M. synoviae. This protein is highly conserved amongst the M. synoviae strains and is strongly immunogenic.

During the sample incubation M. synoviae-specific antibodies bind to the immobilized antigen. The anti-IgY-HRP conjugate detects antibodies bound to the antigen. The color reaction is started by adding the substrate solution and stopped after 10 minutes. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of anti-M. synoviae antibodies in the sample.

 

Procedure

Serum and plasma samples can be tested after dilution.

Samples are incubated for 30 minutes with the test plate and if antibodies to M. synoviae are present, they will bind to the antigen on the plates. Unbound material is removed by rinsing.

The anti-IgY-HRP conjugate is added. It contains an HRP-labeled, anti-IgY-specific polyclonal antibody. Unbound conjugate is rinsed out.

The colour reaction is started by adding the TMB Substrate Solution and stopped after 10 minutes. The optical density (OD) is measured using a spectrophotometer. The results are easy to interpret.

Applications

flocktype Mycoplasma Ms Ab is for the detection of antibodies to Mycoplasma synoviae in serum and plasma samples from chickens and turkeys.

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