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flocktype Mycoplasma Mg/Ms Ab

For detecting antibodies to Mycoplasma gallisepticum and Mycoplasma synoviae in poultry samples
  • Highly sensitive and specific indirect ELISA
  • Suitable for serum and plasma samples
  • User-friendly test protocol with ready-to-use reagents
  • Results in less than 1.5 hours
flocktype Mycoplasma Mg/Ms Ab is an indirect ELISA for screening of avian mycoplasmas (Mycoplasma gallisepticum and Mycoplasma synoviae) in serum and plasma samples from chickens and turkeys.
Cat No./ID: 274803
flocktype Mycoplasma Mg/Ms Ab
5 Test plates, Wash Buffer, Sample Diluent, Positive Control, Negative Control, Conjugate, TMB Substrate Solution, Stop Solution
flocktype Mycoplasma Mg/Ms Ab is for veterinary use only.
Performance
flocktype Mycoplasma Mg/Ms Ab is specific for the detection of antibodies to M. gallisepticum (Mg) and M. synoviae (Ms). Tests were run using the flocktype Mycoplasma Mg/Ms Ab in the Deventer International Proficiency Testing Scheme (PTS) ring trial. All 6 positive sera were correctly scored as being positive for Mg or Ms antibodies, as a result of infection (samples 1, 4, 5, 7) or vaccination (samples 2 and 3). The Mycoplasma meleagridis positive serum, as well as the Nobilis vaccinated SPF layer, were correctly tested negative. This vaccine does not induce a humoral immunity, and therefore gives negative results in all commercially available ELISAs. flocktype Mycoplasma Mg/Ms Ab is a reliable tool for detecting antibodies to Mg and Ms in poultry populations.
 
Deventer PTS 2012 sample origin
Sample Titre
1 Ms ATCC 25204 + Ms Associated Amyloidosis strain 30-week-old SPF layers, sample taken at 35 d.p.i.
2 Mg F-strain vaccine in 13-week-old pullets, sample taken at 24 d.p.v.
3 Poulvac Mg (inactivated vaccine) in 4-week-old SPF layers, sample taken at 21 d.p.v.
4 Ms field strain in 4-week-old SPF layers, sample taken at 7 d.p.i.
5 Nobilis Mg S6/85 + Ms ATCC 25204, chronic infection in SPF layers
6 Nobilis Mg S6/85 strain in 4-week-old SPF layers, sample taken at 21 d.p.v.
7 Ms field strain in 64-week-old SPF layers, sample taken at 28 d.p.i.
8 Mm field strain in 3-week-old turkeys, sample taken at 25 d.p.i.

Deventer PTS 2012 results
Sample Run 1 Run 2
S/P Titre Titre group Result S/P Titre Titre group Result
1 1.939  9354  Positive  2.068  10,163  Positive 
2 0.629  2189  Positive  0.652  2295  Positive 
3 2.670  14,133  11  Positive  2.913  15,810  11  Positive 
4 0.634  2212  Positive  0.555  1861  Positive 
5 1.248  5298  Positive  1.292  5538  Positive 
6 0.055  94  Negative  0.066  119  Negative 
7 3.218  17,979  12  Positive  3.301  18,578  13  Positive 
8 -0.009  Negative  -0.019  Negative 
S/P < 0.3 = negative; 0.3 ≤ S/P < 0.4 = suspect; S/P ≥ 0.4 = positive
Principle
The flocktype Mycoplasma Mg/Ms Ab is a highly sensitive and specific solution for the detection of Mg and Ms antibodies in serum and plasma samples from chickens and turkeys. Mycoplasma infections are spread worldwide and cause severe economic losses in poultry farms due to chronic respiratory diseases, decreased growth, and reduced egg production. Morbidity and mortality can vary widely and depend on environmental conditions (e.g., stress, coldness) and secondary infections (other mycoplasma species, bacteria, or viruses).

M. gallisepticum
infections can cause chronic respiratory diseases in chicken and sinusitis in turkeys. Infection with M. synoviae leads to subclinical disease of the upper respiratory tract and even to synovitis, tendovaginitis, and bursitis. flocktype Mycoplasma Mg/Ms Ab permits quick and reliable surveillance of poultry flocks for antibodies to Mg and Ms induced by infection or vaccination.
Procedure
flocktype Mycoplasma Mg/Ms Ab is an indirect ELISA. The microtiter plate is coated with inactivated mycoplasma antigen. During the sample incubation, antibodies specific to Mg or Ms bind to the immobilized antigen. Unbound material is removed by rinsing. Serum antibodies bound to the antigen are detected by the horseradish peroxidase (HRP)-conjugated anti-IgY antibody. Unbound conjugate is removed by rinsing.

A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. In the presence of Mg- and Ms-specific antibodies, within the sample, HRP catalyzes a blue color development, which turns yellow after adding the Stop Solution. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of Mg- and Ms-specific antibodies in the sample.
Applications
flocktype Mycoplasma Mg/Ms Ab is an indirect ELISA for screening of avian mycoplasmas (Mg and Ms) in serum and plasma samples from chickens and turkeys.

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