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flocktype IBDV Ab

For detection of antibodies to infectious bursal disease virus in samples from chickens

  • Highly sensitive and specific indirect ELISA for infectious bursal disease virus
  • Suitable for serum and plasma samples
  • User-friendly test protocol with ready-to-use reagents
  • Results in less than 1.5 hours


The flocktype IBDV Ab is a sensitive and specific ELISA for detecting antibodies to infectious bursal disease virus in serum and plasma samples from chickens.

flocktype IBDV Ab is for veterinary use only.

In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: FLI-B 387
Cat No./ID: 274202
flocktype IBDV Ab (2)
For 192 reactions: 2 Test plates (strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Anti-IgY-HRP Conjugate, TMB Substrate Solution, Stop Solution

flocktype IBDV Ab is for veterinary use only.

In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: FLI-B 387



Performance
flocktype IBDV Ab is specific for the detection of antibodies to infectious bursal disease virus (IBDV). Tests were run using the flocktype IBDV Ab Kit in the Deventer International Proficiency Testing Scheme (PTS) ring trial. Sample sets were from vaccinated chickens. Six positive serum samples were correctly scored as being positive for IBDV antibodies (samples 1, 2, 3, 5, 6, and 8). Sample 4 represents a very early phase of vaccination and tested negative for IBDV-specific antibodies. Sample 7 was correctly scored as negative (see the table Deventer ring trial IBDV 2007 results).

Deventer ring trial IBDV 2007 results
Sample  Titre  Expected result  flocktype IBDV Ab Kit result   Origin
 1 4459 Positive  Positive  Gallivac vacc.  10 d.p.v. 
 2  2107  Positive  Positive  Bursine 2 vacc.  14 d.p.v. 
 3 4880  Positive  Positive  D78 vacc.  10 d.p.v. 
 4 400  Positive  Negative  D78 vacc.  7 d.p.v. 
 5 2090  Positive  Positive  Bursine 2 vacc.  10 d.p.v. 
 6 6900  Positive  Positive  Vaccinated & Challenged 
 7 70  Negative  Negative  SPF layers  1 year old 
 8 5263  Positive  Positive  D78 vacc.  14 d.p.v. 

The kit correctly scored 7 of the 8 samples. Due to the early phase of vaccination, sample 4 tested negative for IBDV-specific antibodies.
Principle

Infectious bursal disease is caused by infectious bursal disease virus (IBDV). The virus has an affinity to the bursa of Fabricius in chickens and it gives rise to an acquired B lymphocyte deficiency. The disease has mortality rates of 50 to 90%.

flocktype IBDV Ab is an ELISA (enzyme-linked immunosorbent assay) with ready-to-use colored reagents. The test plate is coated with a recombinant structural protein from the virus. This protein is highly conserved amongst infectious bursal disease virus strains and it is considered to be the major immunogenic peptide.

During the sample incubation, IBDV-specific antibodies bind to the immobilized antigen. The anti-IgY-HRP conjugate detects antibodies bound to the antigen. The color reaction is started by adding the substrate solution and stopped after 10 minutes. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of infectious bursal disease virus antibodies in the sample.

Procedure

Serum and plasma samples can be tested after dilution.

Samples are incubated for 30 min with the test plate. If antibodies to infectious bursal disease virus are present, they will bind to the antigen on the plates. Unbound material is removed by rinsing.

The anti-IgY-HRP conjugate is added. Unbound conjugate is rinsed out.

The colour reaction is started by adding the TMB Substrate Solution and stopped after 10 minutes. The optical density (OD) is measured using a spectrophotometer. The results are easy to interpret.

Applications

flocktype IBDV Ab is for the detection of antibodies to infectious bursal disease virus in serum and plasma samples from chickens.

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Safety Data Sheets (1)
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