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flocktype AIV Ab

For detection of antibodies to influenza A virus in samples from poultry

  • Sensitive and specific indirect ELISA for avian influenza A virus
  • Suitable for serum and plasma samples
  • User-friendly test protocol with ready-to-use reagents
  • Results in less than 1.5 hours



flocktype
AIV Ab is a sensitive and specific ELISA for detecting antibodies to avian influenza A virus in serum and plasma samples from chickens and turkeys. It enables rapid and reliable testing of poultry flocks for the virus.

flocktype AIV Ab is for veterinary use only.

In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: FLI-B 435

Cat No./ID: 274012
flocktype AIV Ab (2)
For 192 reactions: 2 Test plates (strips), Wash Buffer, Sample Diluent, Positive Control, Negative Control, Anti-IgY-HRP Conjugate, TMB Substrate Solution, Stop Solution

flocktype AIV Ab is for veterinary use only.

In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: FLI-B 435



0
Sensitivity of flocktype AIV Ab.

An AIV-positive population of 22 serum samples of experimentally infected chickens was tested using flocktype AIV Ab. Serum samples were tested 0, 3, 6, and 19 days-post-infection. Samples with an S/P ratio (ratio sample/positive control) above 0.3 indicate the sample is positive for antibodies to AIV. Six days-post-infection all samples tested positive, providing a diagnostic sensitivity for this sample set of 100%.

1
Specificity of flocktype AIV Ab.

An AIV-negative population of 371 serum samples was tested using flocktype AIV Ab. An S/P (ratio sample/positive control) below 0.3 indicates the sample is negative for antibodies to AIV. All samples tested negative, providing a diagnostic specificity for this sample set of 100%.

Performance
flocktype AIV Ab is specific for the detection of antibodies to avian influenza A virus (AIV). A negative population of 371 serum samples was tested. All of the samples tested negative, providing a diagnostic specificity for this sample set of 100% (see the figure Specificity of flocktype AIV Ab). A positive population of 22 serum samples of experimentally infected chickens was tested. All of the samples tested positive 6 days-post-infection, providing a diagnostic sensitivity for this sample set of 100% (see the figure Sensitivity of flocktype AIV Ab). In a separate study, 33 serum samples, provided by the German NRL for avian influenza (OIE and National Reference Laboratory for Avian Influenza / Fowl Plague [AI]), were tested with the flocktype AIV Ab and all samples tested positive confirming the diagnostic sensitivity for this sample set of 100%.
Principle

Avian influenza is caused by various strains of influenza A virus. It infects wild fowl as well as domestic poultry. Influenza A virus strains are classified as low pathogenic or highly pathogenic. Highly pathogenic strains belong to subtypes H5 or H7 and can cause the severe systemic symptoms known as bird flu or avian flu.

flocktype AIV Ab is an ELISA (enzyme-linked immunosorbent assay) with ready-to-use colored reagents. The test plate is coated with a recombinant structural protein from the virus. This protein is highly conserved amongst avian influenza A virus strains and strongly immunogenic. Thus, all subtypes of influenza A viruses can be detected.

During the sample incubation, influenza A virus-specific antibodies bind to the immobilized antigen. The anti-IgY-HRP conjugate detects antibodies bound to the antigen. The color reaction is started by adding the substrate solution and stopped after 10 minutes. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of anti-influenza A virus antibodies in the sample.

Procedure

Serum and plasma samples can be tested after dilution.

Samples are incubated for 30 minutes with the test plate and if antibodies to influenza A virus are present, they will bind to the antigen on the plates. Unbound material is removed by rinsing.

The polyclonal anti-IgY-HRP conjugate is added. Unbound conjugate is rinsed out.

The colour reaction is started by adding the TMB Substrate Solution and stopped after 10 minutes. The optical density (OD) is measured using a spectrophotometer. The results are easy to interpret.

Applications

flocktype AIV Ab is for the detection of antibodies to influenza A virus in serum and plasma samples from chickens and turkeys.

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Safety Data Sheets (1)
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