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cador C. burnetii PCR Reagent

For the identification of Coxiella burnetii DNA
  • A ready-to-use system for real-time PCR
  • Highly sensitive and specific identification of C. burnetii DNA
  • Internal Control to monitor PCR inhibition
  • A full license for PCR without additional costs

 

The cador C. burnetii PCR reagent is a highly sensitive and specific assay to identify Coxiella burnetii DNA in biological samples.

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Cat No./ID: 285515
cador C. burnetii Reagent (96)
For 96 reactions: cador PCR + IC Reagents, cador Coxiella reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease. Regulatory requirements vary by country; product may not be available in your geographic area.



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Efficient and precise identification of C. burnetii DNA over a wide linear range.
Duplex real-time PCR was carried out using the cador C. burnetii PCR Reagent and the Rotor-Gene Q according to the supplied protocol. C. burnetii DNA was serially diluted as indicated in the figure. Each concentration was analyzed in triplicate. [A] Amplification plots for C. burnetii DNA (one replicate is shown for each template dilution). [B] Amplification plots for IC DNA (one replicate is shown for each template dilution). [C] The log template amount was plotted versus mean CT value, demonstrating a high efficiency, linearity, and precision in identification of C. burnetii DNA. Error bars represent ± 1 SD of 3 replicates.
Performance
The cador C. burnetii Reagent contains reagents and enzymes for the specific and efficient amplification of highly conserved regions of the C. burnetii genome. Inhibition and other malfunctions of PCR are determined by measuring the fluorescence signal in the yellow channel via amplification of the Internal Control (IC), which does not influence the amplification of the analytical PCR for C. burnetii. An external positive control (C. burnetii Control DNA, containing the targeted C. burnetii DNA) is also supplied. C. burnetii DNA is detected efficiently and precisely over a wide linear range (see the figure Efficient and precise identification of C. burnetii DNA over a wide linear range).
Principle
Pathogen identification by the polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In real-time PCR, the amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows detection of the accumulating product without having to re-open the reaction tubes afterward, which reduces the risk of cross contamination.
 
The cador C. burnetii PCR Reagent contains a primer-probe set specific for a highly conserved region of the C. burnetii genome. The reagent also includes a heterologous amplification system as an internal control to ensure the correct interpretation of negative results, and an external positive control. Real-time PCR detection is carried out on a real-time PCR cycler, such as the Rotor-Gene Q.
Procedure
Bacterial DNA can be manually isolated from samples using the QIAamp cador Pathogen Mini Kit. The isolated DNA is ready for use in real-time PCR with the cador C. burnetii PCR Reagent on a real-time PCR cycler, such as the Rotor-Gene Q.
Applications
The cador C. burnetii PCR Reagent is designed to identify Coxiella burnetii DNA in biological samples using polymerase chain reaction (PCR).

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