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Gentra Puregene Yeast/Bact. Kit

For purification of archive-quality DNA from yeast and Gram-positive or Gram-negative bacteria
  • Archive-quality DNA for long-term storage
  • Reproducible DNA purification
  • A complete solution for sample-to-storage purification
  • Convenient, scalable purification procedure

The Gentra Puregene Yeast/Bact. Kit enables purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

Cat No./ID: 158567
Gentra Puregene Yeast/Bact. Kit (200 ml)
$554.00
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For purification of archive-quality DNA from 200 ml bacterial cultures or 400 ml yeast cultures: Cell Suspension Solution, Lytic Enzyme Solution, RNase A Solution, and Reagents
The Gentra Puregene Yeast/Bact. Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
1
Reproducible purification of archive-quality DNA.
DNA was purified from yeast cultures using the Gentra Puregene Yeast/Bact. Kit. Purified DNA (100 ng) was analyzed by agarose gel electrophoresis. M1: Uncut Lambda DNA; M2: Lambda HindIII marker.
Performance
Archive-quality DNA

Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. The Gentra Puregene Yeast/Bact. Kit removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb (see figure "Reproducible purification of archive-quality DNA").

Principle
Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.
Procedure
The simple Puregene procedure uses a modified salting-out precipitation method for purification of DNA (see flowchart "Puregene DNA procedure"). No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.
Applications

DNA purified using the Gentra Puregene Yeast/Bact. Kit is highly stable and suited for use in a wide range of applications, such as:

DNA archiving
PCR
SNP analysis
Southern blotting
Features
Specifications
Applications PCR, restriction digest, Southern analysis, SNP analysis
Elution volume 50 µl (varies)
Format Scalable
Main sample type Gram+ and Gram- Bacteria, Yeast
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Bacterial DNA / Yeast DNA
Sample amount 0.5-1.5*10e9 cells / 1-2*10e8 cells
Technology Modified salting-out precipitation method
Time per run or per prep 25 - 60 minutes (+ DNA rehydration)
Yield 17-50 µg/ml culture / 4.5 µg/ml culture

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Kit Handbooks (2)
For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, buccal cells, body fluids, cultured cells, tissue, mouse tail, yeast, bacteria
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以下サンプルから長期保存に安定なゲノムDNAの精製: ヒト全血; 骨髄液 ; バフィーコート; 口腔細胞; 体液; 培養細胞; 組織; マウス尾; 酵母; バクテリア
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Instrument Technical Documents (1)
Supplementary Protocols (4)
This protocol is designed for purification of DNA from 300 μl samples of whole blood using the Gentra Puregene Yeast/Bact. Kit.
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This protocol is designed for purification of DNA from 1 g samples of soil using the Gentra Puregene Yeast/Bact. Kit.
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This protocol is designed for purification of DNA from 0.5 or 1 ml samples of Gram-positive bacteria culture medium using the Gentra Puregene Yeast/Bact. Kit.
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This protocol is designed for purification of DNA from 5 ml samples of Gram-positive bacteria culture medium using the Gentra Puregene Yeast/Bact. Kit.
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