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QIAamp One-For-All Nucleic Acid Kit

For automated purification of viral DNA and RNA or genomic DNA from a variety of sample types
  • Rapid isolation of high-quality, viral DNA and RNA or genomic DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors
  • Flexibility with multiple sample types in the same run

The QIAamp One-For-All Nucleic Acid Kit provides automated purification of viral nucleic acids or genomic DNA on the BioRobot MDx or the BioRobot Universal System using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run. The QIAamp One-For-All Nucleic Acid Kit enables processing of different samples (e.g., viral RNA and DNA from serum, genomic DNA from blood) in the same run.

Cat No./ID: 965672
QIAamp One-For-All Nucleic Acid Kit (12)
$9,704.00
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For use on the BioRobot MDx. Includes: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, Elution Microtubes CL, caps, S-Blocks, carrier RNA
The QIAamp One-For-All Nucleic Acid Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
Reliable purification of EBV DNA from cells.
Viral DNA was purified from serial dilutions of EBV-infected Raji cells (50,000–10 cells/ml) using the QIAamp One-For-All Nucleic Acid Kit on the BioRobot MDx. Purified viral DNA was analyzed by quantitative real-time PCR. (Data kindly provided by M.J. Bankowski, S.W. Belzer, B.D. Lembke, and S.M. Anderson, ViroMed [LabCorp] Laboratories, Minnetonka, MN, USA.)
1
Efficient purification of viral DNA from whole blood.
Viral DNA was purified from a QCMD panel of CMV-positive whole blood samples and a set of negative human samples using the QIAamp One-For-All Nucleic Acid Kit on the BioRobot MDx. [A] All QCMD samples were identified correctly by quantitative real-time PCR. (Negative samples QCMD 05-03 and 05-08 tested negative, as expected.) [B] All negative human samples tested negative. QS4: positive control (quantitation standard).
2
Reliable purification of viral DNA over a range of viral loads.
Viral DNA was purified from serial dilutions (2000-50 copies/ml) of CMV-positive sample QCMD 05-07 using the QIAamp One-For-All Nucleic Acid Kit on the BioRobot MDx. [A] Viral DNA was analyzed by quantitative real-time PCR. The limit of detection was lower than the lowest dilution (<50 copies/ml). [B]Internal control signals for all samples demonstrate efficient DNA recovery for all samples.
3
Repeatable purification of viral nucleic acids.
Viral DNA was purified from serial dilutions of adenovirus using the QIAamp One-For-All Nucleic Acid Kit on the BioRobot MDx on 2 different days. Purified viral DNA was analyzed by quantitative real-time PCR. Results from both days were comparable, demonstrating consistent yields. Neg: negative control. (Data kindly provided by M.J. Bankowski, S.W. Belzer, B.D. Lembke, and S.M. Anderson, ViroMed [LabCorp] Laboratories, Minnetonka, MN, USA.)
Performance

The QIAamp One-For-All Nucleic Acid Kit can be used for a wide range of sample types, including, for example, cells (see figure "Reliable purification of EBV DNA") and whole blood (see figure "Efficient purification of viral DNA").

Consistent, high yields

The kit provides consistent high yields of viral nucleic acids over a wide range of viral loads (see figure "Reliable purification of viral DNA"). Automation ensures a high degree of standardization for consistent, repeatable results (see figure "Repeatable purification of viral nucleic acids").  DNA and RNA purified using the QIAamp One-For-All Nucleic Acid Kit can be used in a range of downstream applications, including:

PCR and quantitative real-time PCR and RT-PCR
Infectious disease research
Principle

The QIAamp One-For-All Nucleic Acid Kit simplifies isolation of viral RNA and DNA from a wide range of sample types with a fast vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit.

QIAamp sample preparation technology is fully licensed.

Procedure

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities. Viral nucleic acids, genomic DNA, and cellular RNA are eluted, and ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Applications

The QIAamp One-For-All Nucleic Acid Kit is designed to allow automated processing of multiple sample types in the same run. Sample types include:

Whole blood
Plasma and serum
CSF
Swabs
Urine
Features
Specifications
Applications PCR, qPCR, real-time PCR
Elution volume 75 µl
For automated processing BioRobot MDx workstation or BioRobot Universal platform
Format 96-well plates
Main sample type Cell-free body fluids like plasma, serum, CSF; whole blood; swabs; urine
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA and RNA, genomic DNA
Sample amount 265 µl
Samples per run; throughput 32-96 samples per run
Technology Silica technology
Time per run or per prep <2.5 hours
Yield >90% recovery

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Kit Handbooks (1)
For automated purification of nucleic acids from a wide range of sample types using the BioRobot MDx or BioRobot Universal System
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