QIAsymphony DNA Investigator Kit
For automated purification of DNA from 1–96 samples
- Purification from forensic and human identity samples
- More efficient yields from trace casework samples
- Higher signal-to-noise ratios
- Novel, prefilled reagent cartridges for ease of use
- High reproducibility through standardized processing
The QIAsymphony DNA Investigator Kit enables automated purification of genomic DNA from 1–96 samples, such as swabs, filters, casework or crime-scene samples, and blood on the QIAsymphony SP. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.
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QIAsymphony DNA Investigator Kit (192)
For 192 preps of 200 µl each from casework and reference samples: includes 2 reagent cartridges and enzyme racks and accessories
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931436
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The QIAsymphony DNA Investigator Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Efficient processing of magnetic particles.|Prefilled, sealed reagent cartridges.|
The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.
|Reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes, and are labeled with 2D bar codes. Partially used cartridges can be resealed for later use giving you complete cost control. |
Performance
The QIAsymphony DNA Investigator Kit enables highly efficient purification of DNA, even from very small amounts of sample material. The combination of sample input volumes of up to 1000 µl with elution volumes as low as 30 µl enables highly sensitive detection of DNA from a wide range of sample types, including diffuse stains.
Principle
The QIAsymphony DNA Investigator Kit combines the speed and efficiency of silica-based purification of genomic and mitochondrial DNA with the convenient handling of magnetic particles (see figure " Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the "Reagents and Consumables" drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. For increased flexibility, DNA can be eluted in volumes of between 30 and 400 µl of water or Buffer ATE (modified TE buffer).
Procedure
The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 μl to 400 μl of water or modified TE Buffer (Buffer ATE), depending on the protocol.
Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 μl, 500 μl or 1000 μl.
There are two different kinds of DNA purification protocols, which can be used in conjunction with the pretreatment protocols. Casework protocols purify genomic and mitochondrial DNA from 200 μl, 500 μl or 1000 μl of lysate obtained from the sample lysis procedures detailed in the corresponding pretreatment protocols. DNA can be eluted in 30–200 μl of either water or modified TE Buffer (Buffer ATE). Reference protocols purify DNA from database samples, such as buccal swabs or dried blood. DNA can be eluted in 100–400 μl of Buffer ATE. The purified DNA is ready to use in downstream applications.
| Reference |
200, 500 μl |
No |
100–400 μl |
| Casework |
200, 500, 1000 μl |
No |
100–200 μl |
| Casework ADV |
200, 500, 1000 μl |
Yes |
100–200 μl |
| Casework HE |
200, 500, 1000 μl |
No |
30–80 μl |
| Casework HE ADV |
200, 500, 1000 μl |
Yes |
30–80 μl |
Applications
The high-quality DNA obtained using the QIAsymphony DNA Investigator Kit is suitable for direct use in forensic, environmental, biodefense, biosecurity, and other applied-testing applications, such as:
- Genotyping, including fingerprinting and paternity analysis
- DNA purification from trace samples (e.g., crime-scene)
- DNA extraction from filters used in environmental testing
- Routine analysis of reference samples
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Feature
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Specifications
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Applications
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STR analysis, (real-time) PCR
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Elution volume
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Reference samples: 100-400 µl; Casework samples: 30- 200 µl
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Input volume
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Reference samples: 200 or 500 µl lysate; Casework samples: 200, 500 or 1000 µl lysate
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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Genomic DNA, mitochondrial DNA
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Sample type
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Wide range of forensic and human-identity sample materials
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Technology
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Magnetic particle technology
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Yield
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< 3 µg
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For use with software version 4.0
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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High-throughput DNA sample prep using the QIAsymphony SP instrument: An overview of workflow optimization at Bode Cellmark Forensics.
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Application/Protocol Documents
Instrument Technical Documents
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
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This protocol has been adapted from the pretreatment of sexual assault samples and is for the lysis and extraction of DNA from a sperm pellet after differential separation.
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