Large-Scale Plasmid Purification — Product Overview

QIAGEN offers a wide range of products for large-scale plasmid preps
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For large-scale purification of molecular biology or transfection grade plasmid DNA, trust QIAGEN's wide range of products! Speed, superior quality, high purity, and unmatched yields are just some of the benefits that can be experienced with QIAGEN's large-scale plasmid purification products. Efficient and reliable endotoxin removal ensures success in transfection experiments. Streamlined protocols and additonal features such as reagents for visualization of optimum lysis conditions, assure maximal convenience.

No matter what your downstream application, we've got a product that best matches your needs!

 

 

View our helpful selection guide below to choose the right product for your downstream application:


 
Large-scale plasmid purification kits from QIAGEN
Application HiSpeed Plasmid Kits QIAGEN Plasmid/
QIAfilter Plasmid Kits
QIAGEN Large- Construct Kit CompactPrep Plasmid Kits QIAGEN Plasmid Plus Kits Endofree 
Plasmid Kits 
Ultrapure Plasmid DNA Prep. Services
  Large-scale plasmid kits
in spin-column format
    Preclinical-scale
(1–100 mg)
Transfection of primary/sensitive cells         X X X
Gene silencing X X X X X X X 
Microinjection X X X   X X X  X
Research on gene therapy           X X X 
Transfection of most cells* X X X   X  X X X
Demanding enzymatic modifications X X X X X X X X
Library constructions (BACs and cosmids) X X X X X X X
Transfection of robust cells* X X   X  X X X X
In vitro transcription X X   X X X X X
In vitro translation X X    X X  X X X
High-quality sequencing X X X  X  X  X X X
Cloning X  X  X  X X  X X X
Probe generation X  X X  X X X X X
High-throughput sequencing X X X X X X X X
PCR X X  X  X X  X X X
Restriction digestion X  X  X X X X X X
Transformation X X  X X  X  X X X
X: Recommended kit; X: DNA grade or kit format can be used but may not be optimal.
This table indicates minimum DNA purity requirements. Higher DNA purity may improve performance or be required for some applications.
* Transfection of sensitive cells may require a higher DNA grade.
Long reads and difficult or heterogeneous templates may require a higher DNA purity.

Top products
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For use with the Ultrapure 500 Resin
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For fast purification of up to 750 µg molecular biology grade plasmid or cosmid DNA
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For fast purification of up to 200 µg molecular biology grade plasmid DNA
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QIAGEN Large-Construct Kit
For purification of up to 50 μg BAC, PAC, and P1 DNA or up to 200 μg cosmid DNA, free of genomic DNA
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For isolation of up to 100 µg high-molecular-weight DNA from a wide range of samples
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For isolation of up to 500 µg high-molecular-weight DNA from a wide range of samples
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For isolation of up to 20 µg high-molecular-weight DNA from a wide range of samples
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