Innovative NGS Library Construction Technology

 
Dec 8, 2016 1:00 PM–2:00 PM (EST)
Duration: 1hrs

Next-generation sequencing (NGS) is a driving force for numerous new and exciting applications, including cancer research, stem cell research, metagenomics, population genetics, medical research and single cell analysis. While NGS technology is continuously improving, library preparation remains one of the biggest bottlenecks in the NGS workflow and includes several time-consuming steps that can result in considerable sample loss and the potential to introduce handling errors. Moreover, conducting single-cell genomic analysis using NGS methods has traditionally been challenging since the amount of genomic DNA present in a single cell is very limited.

 

In this webinar, we will provide an overview of methods for the preparation of high-quality DNA libraries directly from high-molecular-weight DNA using innovative enzymatic fragmentation that is integrated in the DNA end-repair step. We will discuss complete PCR-free solutions for whole genome and whole transcriptome sequencing from isolated single cells and low amounts of genomic DNA or RNA. Additionally, we will provide details on a time-saving, one-tube library preparation protocol that does not require extra nucleic acids fragmentation and sample cleanup between steps, minimizing starting material loss and cross-contamination risk, thereby increasing the resolution of NGS data.


Ioanna Andreou

Dr. Ioanna Andreou is a Senior Scientist at QIAGEN and is responsible for developing technologies for single cell analysis. Dr. Andreou received her Ph.D. from the University of Freiburg, focusing on early cancer detection based on autoimmune response. She joined QIAGEN after a postdoctoral fellowship at the University of Cologne in 2002, and has held multiple positions in R&D in the area of molecular diagnostics, modification and amplification, and cell biology. She has published extensively in the area of gene silencing and holds multiple patents in this field.