Six Strains in One Go – Detect the E. coli 'Big Six' in a New AOAC-RI-Certified Workflow


Industrial food safety testing is important for safeguarding consumers against foodborne diseases, and the presence of harmful E. coli in food is still a major concern. Recent regulations require testing for the virulence factors stx1/stx2, eae and O157, and the presence of these necessitates further testing for adulterant O serotypes. But obtaining reliable food safety testing results relies equally on highly efficient sample preparation and sensitive and accurate sample analysis.

We will discuss an AOAC-RI PTM validated one-for-all workflow for DNA-based screening of E. coli virulence factors and E. coli STEC O-types and how the presence of E. coli O157:H7 or non-O157 serotypes can be determined using the same equipment and workflow used for detection of Salmonella and Listeria spp. Preparing and analyzing DNA samples from food pathogen cultures using the QIAsymphony RGQ instrument and mericon pathogen detection kits provides high process safety and standardization and minimizes hands-on time for fast and reliable results.

Dr. Marcia Armstrong

Marcia Armstrong
Dr. Marcia Armstrong obtained her doctorate in nutrition from the Harvard School of Public Health. After a post-doctoral research fellowship in the Gastroenterology Department at the Brigham and Women's Hospital, a teaching affiliate of Harvard Medical School, she was an Assistant Professor in the Department of Physiology at the University of Massachusetts Medical School. She has been with QIAGEN for 20 years and is currently Global Scientific Affairs Manager with the food safety testing team.