GeneRead DNAseq Targeted Panels V2

For targeted enrichment of biologically and clinically relevant panels using any of 15 laboratory-verified primer sets prior to NGS

Outstanding sequencing performance
As little as 10 ng DNA needed
Compatible with many samples types including FFPE samples
Can be used on any sequencing platform
Clinically and biologically focused content

GeneRead DNAseq Targeted Panels V2 are the simplest tools for analyzing the genetic variants of a focused panel of genes via next-generation sequencing. Each panel consists of multiplex PCR primer sets to amplify exonic regions of a thoroughly researched panel of biologically and clinically relevant and disease-focused genes. GeneRead DNAseq Targeted Panels V2 can also be customized to include genes or other genomic regions tailored to your specific NGS project needs. Our high-quality primer design and targeted enrichment chemistry provide high design coverage, specificity, and uniformity, which are essential for detecting low-frequency variants in your precious samples.

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Available Products

Product	Product no.	Cat. no.	List price:
Human Breast Cancer Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-001X	varies	configure
Human Colorectal Cancer Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-002X	varies	configure
Human Myeloid Neoplasms Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-003X	varies	configure
Human Liver Cancer Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-004X	varies	configure
Human Lung Cancer Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-005X	varies	configure
Human Ovarian Cancer Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-006X	varies	configure
Human Prostate Cancer Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-007X	varies	configure
Human Gastric Cancer Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-008X	varies	configure
Human Cardiomyopathy Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-009X	varies	configure
Human Carrier Testing Panel Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181901	NGHS-011X	varies	configure
Human Cancer Predisposition Panel Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181901	NGHS-013X	varies	configure
Human Clinically Relevant Tumor Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-101X	varies	configure
Human BRCA1 and BRCA2 Panel Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-102X	varies	configure
Human Tumor Actionable Mutations Panel Sets of 1 tube containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181900	NGHS-201X	varies	configure
Human Comprehensive Cancer Panel Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes show details	181901	NGHS-501X	varies	configure

GeneRead DNAseq Targeted Panels V2 are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

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GeneRead DNAseq Targeted Panel V2 workflow.|Multiplex PCR-enabled target enrichment of genomic regions of interest|

Genomic DNA (the QIAamp DNA Mini Kit, QIAamp DNA FFPE Tissue Kit, or GeneRead DNA FFPE Kit are recommended for extraction) can be quantified and qualified using the GeneRead DNA QuantiMIZE System. The GeneRead DNAseq Targeted Panels V2 are then used for targeted enrichment. Following target enrichment, construct your NGS library, quantify and quality-control using the GeneRead Library Quantification System, perform NGS, and analyze the data using the QIAGEN NGS Data Analysis Web Portal.|The principle of the GeneRead DNAseq V2 System is to employ overlapping primer sets across the exonic portions of a gene or genes to maximize target coverage and minimize nonspecific amplification.|

Performance

The performance of GeneRead DNAseq Targeted Panels V2 is assessed by 3 criteria: design coverage, specificity, and coverage uniformity. Greater than 95% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 95% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 90% of targeted bases covered at >20% median sequencing depth, ensures high-quality variant calls.

Outstanding experimental performance metrics
Type	Panel name	Coverage (%)	Specificity (%)	Uniformity (%)
Solid tumors	Tumor Actionable Mutations	100.0	98.2	91
Solid tumors	Clinically Relevant Tumor	98.1	95.3	90
Hematologic malignancies	Myeloid Neoplasms	98.1	97.4	94
Disease-specific	Breast Cancer	98.2	96.8	91
	Colorectal Cancer	98.7	98.3	95
	Liver Cancer	99.0	96.4	96
	Lung Cancer	97.5	98.1	90
	Ovarian Cancer	98.9	96.6	96
	Prostate Cancer	98.4	97.3	94
	Gastric Cancer	98.1	98.5	93
	Cardiomyopathy	96.3	96.7	87
Comprehensive	Carrier Testing	97.5	97.9	91
	Cancer Predisposition	98.3	96.8	93
	Comprehensive Cancer	98.0	97.7	92
Gene-specific	BRCA1 and BRCA2	100.0	99.0	97

Principle

The GeneRead DNAseq Targeted Panel System employs overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see Multiplex PCR-enabled target enrichment of genomic regions of interest). Overlapping primer sets are divided into an appropriate number of pools to maximize specificity. Following amplification and purification, enriched regions from each sample are pooled together, yielding one library preparation for each sample.

Specifications of GeneRead DNAseq Targeted Panels V2
Type	Panel name	# Genes	# Amplicons	Target region (bases)
Solid tumors	Tumor Actionable Mutations	8	118	7104
Solid tumors	Clinically Relevant Tumor	24	602	39603
Hematologic malignancy	Myeloid Neoplasms	50	2536	236319
Disease-specific	Breast Cancer	44	2915	268621
	Colorectal Cancer	38	1954	182851
	Liver Cancer	33	2052	191170
	Lung Cancer	45	3586	332999
	Ovarian Cancer	32	2021	198058
	Prostate Cancer	32	1837	167195
	Gastric Cancer	29	2377	222333
	Cardiomyopathy	58	2657	249727
Comprehensive	Carrier Testing	157	6943	664735
	Cancer Predisposition	143	6582	620318
	Comprehensive Cancer	160	7951	744835
Gene-specific	BRCA1 and BRCA2	2	250	21472

Procedure

GeneRead DNAseq Targeted Panels V2 are part of a total workflow for targeted next-generation sequencing (see GeneRead DNAseq Targeted Panels V2 workflow). Simply extract DNA from your samples (the QIAamp DNA Mini Kit, the QIAamp DNA FFPE Tissue Kit or the GeneRead DNA FFPE Kit are recommended), quantify and qualify your DNA sample with the GeneRead DNA QuantiMIZE system, and then use the GeneRead DNAseq Targeted Panels V2 in combination with GeneRead DNAseq Panel PCR Kit V2 to perform targeted enrichment using multiplex PCR. Once targets have been enriched, construct the NGS library and use the GeneRead DNAseq Library Quant Array to quantify and perform quality control. Perform NGS. Multiple samples can be analyzed with a single NGS run. Use the Multiplexing Capacity Calculator (see the Resources tab) to determine the optimal number of samples and coverage depth for each NGS run. Analyze your data using the QIAGEN NGS Data Analysis Web Portal or CLC Bio pipelines, and interpret detected variants using the Ingenuity Variant Analysis platform.

Applications

GeneRead DNAseq Targeted Panels V2 are highly suited for enriching target genes specific for various cancers, inherited diseases, neurological diseases, cardiomyopathy, developmental disorders, metabolic disorders and many other areas of biomedical research to determine genetic variants through NGS.

Application Notes

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	Development of an Individual Identity SNP panel and workflow for paternity and forensic applications	Show details

Brochures & Guides

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	Complete NGS Product Configurator GeneRead Panels and NGS product configurator guide	Show details
	GeneRead DNAseq Targeted Panels V2 For targeted enrichment prior to next-generation sequencing	Show details

Analysis Software

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	CLC Genomics Workbench For analyzing, comparing, and visualizing next-generation sequencing data	View
	QIAGEN NGS Data Analysis Web Portal For basic analysis of NGS data using the GeneRead DNAseq panels V2; acceptable file formats include FASTQ or BAM	View
	Ingenuity Variant Analysis For identifying disease-causing variants from human whole genome, exome, and gene panel next-generation sequencing studies	View

Scientific Posters

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	Targeted Sequencing for Accurate Mutation Detection at the Single-Cell Level	Show details
	Single cell mutation detection with multiplex PCR-based targeted enrichment	Show details

Kit Handbooks

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	GeneRead DNAseq Targeted Panels V2 Handbook 06/15 All-bead protocol for targeted enrichment prior to next-generation sequencing	Show details

Safety Data Sheets

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	MSDS GeneRead DNAseq Targeted Panels V2
	MSDS GeneRead DNAseq Targeted Panels High-Content V2

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