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DNeasy PowerSoil Kit

For the isolation of microbial genomic DNA from all soil types
  • Fast and easy protocol isolates high-quality DNA from up to 250 mg samples in just 30 minutes
  • Inhibitor Removal Technology removes PCR inhibitors found in tough sample types
  • Optimized to isolate DNA from difficult even the most difficult environmental samples

Isolate DNA from any soil or environmental sample source with the DNeasy PowerSoil Kit. The DNeasy PowerSoil Kit can isolate microbial genomic DNA from even tough compost, sediment or manure samples using Inhibitor Removal Technology (IRT). IRT eliminates humic acid content leaving highly pure DNA for downstream applications analysis. PCR analysis can detect DNA from a variety of organisms including fungi, algae, Gram (+/-) bacteria (e.g., Bacillus subtilis, Bacillus anthracis), actinomycetes (e.g., Streptomyces) and nematodes.

Isolation of DNA using the DNeasy PowerSoil Kit can be automated on the QIAcube Connect.

DNeasy PowerSoil Kit was formerly sold by MO BIO as PowerSoil DNA Isolation Kit.

<p style="padding: 20px; border: 1px solid #e7edf4; background-color: #f2f8ff;">DNeasy PowerSoil Pro Kit &ndash; higher DNA yields, faster protocol, superior microbial diversity.<a href="https://www.qiagen.com/shop/sample-technologies/dna/genomic-dna/dneasy-powersoil-pro-kit" class="blueButton" id="ecommerce" style="color: #ffffff;" target="_blank">See new kit</a></p>

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Cat No./ID: 12888-100
DNeasy PowerSoil Kit (100)
$862.00
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For the isolation of microbial genomic DNA from all soil types
Cat No./ID: 12888-100-1
Solution C1 (6.6 ml)
$31.80
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DNeasy PowerSoil Isolation Kit Components
Cat No./ID: 12888-100-2
Solution C2 (2 x 15 ml)
$89.10
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DNeasy PowerSoil Isolation Kit Components
Cat No./ID: 12888-100-3
Solution C3 (2 x 15 ml)
$162.00
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DNeasy PowerSoil Isolation Kit Components
Cat No./ID: 12888-100-4
Solution C4 (2 x 72 ml)
$175.00
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DNeasy PowerSoil Isolation Kit Components
Cat No./ID: 12888-100-5
Solution C5 (2 x 30 ml)
$95.40
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DNeasy PowerSoil Isolation Kit Components
Cat No./ID: 12888-100-PBT
PowerBead Tubes, Garnet (100)
$493.00
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DNeasy PowerSoil Isolation Kit Components
Cat No./ID: 12888-50
DNeasy PowerSoil Kit (50)
$471.00
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For the isolation of microbial genomic DNA from all soil types

Product Details

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Figure 1: Isolation of high-quality genomic DNA.
The DNeasy PowerSoil Kit was used to isolate total genomic DNA from nine different sample types. The results were compared with those from soil DNA isolation kits from two other suppliers. All manufacturer’s protocols were followed. 15 µl of eluted genomic DNA was used per lane of a 0.8% TAE agarose gel.
1
Figure 2: Reliable PCR analysis with the DNeasy PowerSoil Kit.
The undiluted DNA elute (1 µl) described in Figure 1 underwent PCR analysis with eubacterial primers. The results are displayed on an 0.8% TAE agarose gel. The DNeasy PowerSoil Kit was the only kit that yielded a PCR product for landfill samples (lanes 1 and 2), lake sediment (lane 6) and mulch topsoil (lane 9). High remaining humic acid content inhibited amplification for DNA isolated with kits from other manufacturers. DNA isolated with the DNeasy PowerSoil Kit had positive amplification in 100% of samples. Other supplier kits had positive amplification in 44% of samples.
Performance


Specifications

Features
Specifications
Binding capacity/yield Up to 20 µg per filter
Format Silica Spin Filter Tubes
Processing Bead beating
Sample size 0.25 g
Sample types Soil, fecal, water, food, insects, swabs with PCR inhibitors
Special feature Bead Type: 0.7 mm garnet
Storage conditions Store at room temperature (15-30°C)
Storage temperature Store at room temperature (15-30°C)
Throughput 1 - 24 samples
Time per run or per prep 30 minutes

Product citations in scientific articles

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