For the removal of PCR inhibitors from purified DNA in just 7 minutes
Faster cleanup of problematic DNA in 7 minutes with efficient secondary protocol
Efficient purification of nucleic acids from any sample source containing challenging impurities
Ready-to-use, high-purity DNA for downstream applications
Clean up problematic DNA in a snap. Using the streamlined DNeasy PowerClean Pro Cleanup Kit’s 7-minute protocol and Inhibitor Removal Technology provides researchers with a novel and proprietary method for cleaning up previously isolated genomic DNA. Regardless of whether the inhibiting substance colors previously processed DNA or not, the DNeasy PowerClean Pro Cleanup Kit will remove both color and inhibitors, such as humic acids, heme, polysaccharides, polyphenols, fluvic acids, lipids and dyes from samples. Resultant, high-purity DNA is ready to use in all downstream applications including qPCR and next-generation sequencing.
The DNeasy PowerClean Pro Cleanup Kit performs well on DNA from any sample source. DNA samples are combined with propriety DNA cleanup reagents to remove inhibitors from the DNA solutions. Using a silica membrane spin column, all DNA, including total genomic DNA is captured. Washing and eluting the DNA from the spin column provides the purified DNA, ready for downstream applications. Percentage recovery varies depending on the level of inhibitors in the DNA that may be influencing the DNA yield measurement. We recommend quantifying DNA via a PicoGreen assay or running DNA on an agarose gel to determine recovery yield. Want to try this solution for the first time? Request a quote for a trial kit.
Purification of DNA using the DNeasy PowerClean Pro Cleanup Kit can be automated on the QIAcube Connect.
DNeasy PowerClean Pro Cleanup Kit was formally sold by MO BIO as PowerClean Pro DNA Clean-Up Kit.
Remove PCR inhibitors from purified DNA in just 7 minutes.
The DNeasy PowerClean Pro Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Figure 2: Improved PCR success with pure DNA.
Samples described in Figure 1 were examined via RT-qPCR with a Bacillus 16S assay (1 µl of undiluted RNA or a 1:10 dilution). RNA samples were successfully amplified, confirming they were free of inhibitors at a difference of approximately 3 cycles between the undiluted and 1:10 dilution.
Figure 1. More pure DNA from samples with high humic content.
DNA samples were cleaned with either the DNeasy PowerClean Pro Cleanup Kit or the Zymo Research OneStepTM PCR Inhibitor Removal Kit.(A) Results were then examined on a 1.2% TAE gel. (B) Side-by-side comparison shows that while samples processed with the DNeasy PowerClean Pro Cleanup Kit were clear, some brown color remained in those processed with the OneStep Kit, showing some residual humic substances may be present. Starting samples were brown and concentration appeared artificially high. Likewise, when looking at the ratios of 260/280 and 260/230, the starting sample had low ratios of both, showing the presence of contaminants including humic substances. Following clean-up, the DNeasy PowerClean Pro Cleanup Kit ratios of 260/280 and 260/230 increased to levels consistent with pure DNA. The concentration of DNA decreased to an average of 76.85 ng/µl. Samples cleaned with the OneStep Kit ratios of 260/280 and 260/230 remained low.
For processing of approximately 10 mg tagged protein: 0.5 units DAPase Enzyme, 30 units Qcyclase Enzyme, 10 units pGAPase Enzyme, 20 mM Cysteamine-HCl (1 ml), Ni-NTA Agarose (10 ml), 20 Disposable Columns