Highly pure plasmid DNA
The QIAcube together with the QIAprep Spin Miniprep Kit enables fully automated purification of up to 20 µg molecular biology grade plasmid DNA. The fully automated purification procedure — including lysis — provides yields of plasmid DNA comparable to the manual procedure (see figure “ DNA yields comparable to the manual procedure”). Purified DNA is suitable for use in routine molecular biology applications, such as fluorescent sequencing, cloning, or transfection of robust cells (see figure “ Reliable sequencing results”).
High-quality genomic DNA
Using the QIAamp DNA Blood Kit, the QIAcube enables purification of reproducible yields of highly pure genomic DNA (see figure “ Reproducible DNA yields”). DNA purified using the QIAcube performs well in sensitive PCR even when large amounts of eluate are used, demonstrating the high purity of the DNA (see figure “ High PCR performance”).
Efficient purification of viral nucleic acids
The QIAcube together with the QIAamp MinElute Virus Spin Kit enables efficient purification of viral nucleic acids. To evaluate the risk of sample-to-sample carryover during and between runs, the QIAamp MinElute Virus Spin procedure was subjected to rigorous testing using an alternating checkerboard setup of negative and highly positive plasma samples (1 x 108 IU/ml of a typical DNA virus). All of the highly positive samples were detected. All negative samples, in the checkerboard runs and the all-negative runs were unresponsive (see table “No sample carryover detected”).
|Run 1 ||19.5 ||X ||19.4 ||X ||18.8 ||X ||X ||19.0 ||X ||19.1 ||X ||19.8 |
|Run 2 ||X ||19.1 ||X ||18.9 ||X ||18.8 ||18.7 ||X ||19.2 ||X ||18.9 ||X |
|Run 3 ||X || ||X ||X ||X ||X ||X ||X ||X ||X ||X ||X |
Pure, high performance RNA
Together with the QIAcube, the RNeasy Mini Kit enables efficient automated purification of total RNA from animal cells, demonstrated by consistent RNA yields (see figure “ Consistent yields of RNA”). The quality of RNA purified using the QIAcube is comparable to the manual procedure (see figure “ Comparison of automated and manual RNA purification”).
The QIAcube and Ni-NTA Spin Kit provide reliable purification of highly pure 6xHis-tagged proteins (see figure “ Highly reproducible protein purification”). Purified proteins are suitable for many applications, including functional investigations, crystallization pre-studies for determination of three-dimensional structure, assays involving protein–protein and protein–DNA interactions, and immunization to produce antibodies.
Efficient cleanup of DNA fragments
The QIAcube makes DNA cleanup easier than ever. Proven QIAquick technology together with optimized protocols enables direct purification of double- or single-stranded DNA fragments, from 100 bp to 10 kb. Primers, nucleotides, enzymes, and other impurities are efficiently removed and recovery of DNA is comparable to the manual procedure (see figure “ High recovery of PCR products”).