text.skipToContent text.skipToNavigation

Cignal Reporter Assay Kits

For rapid, sensitive, and quantitative assessment of signal transduction pathway activation

  • Rapid analysis of signal transduction pathway regulation
  • Exceptional sensitivity and specificity
  • Transfection-ready constructs
  • Includes positive and negative controls
  • Real-time live cell quantification (GFP format)

Cignal Reporter Assays provided in the Cignal Reporter Assay Kit enable rapid, sensitive, and quantitative assessment of signal transduction pathway activation by measuring the activities of downstream transcription factors. Two reporter systems are available: dual-luciferase format and GFP format.

Product Product no. Cat. no. List price:
 
 
Show details 		    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Available Products
61
Product Product no. Cat. no. List price:
Cignal C/EBP Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-001L $592.00
add to cart
Cignal Antioxidant Response Reporter (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-0020G $592.00
add to cart
Cignal Oct4 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-0025L $592.00
add to cart
Cignal CRE Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-002G $592.00
add to cart
Cignal CRE Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-002L $592.00
add to cart
Cignal MTF1 Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-0033G $592.00
add to cart
Cignal Sox2 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-0038L $592.00
add to cart
Cignal E2F Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-003L $592.00
add to cart
Cignal LXR Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-0041L $592.00
add to cart
Cignal p53 Pathway Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-004L $592.00
add to cart
Cignal ERE Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-005L $592.00
add to cart
Cignal GRE Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-006L $592.00
add to cart
Cignal HIF Pathway Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-007L $592.00
add to cart
Cignal ISRE Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-008G $592.00
add to cart
Cignal ISRE Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-008L $592.00
add to cart
Cignal GAS Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-009L $592.00
add to cart
Cignal SRE Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-010G $592.00
add to cart
Cignal SRE Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-010L $592.00
add to cart
Cignal AP1 Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-011G $592.00
add to cart
Cignal AP1 Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-011L $592.00
add to cart
Cignal Myc Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-012L $592.00
add to cart
Cignal NFkB Pathway Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-013G $592.00
add to cart
Cignal NFkB Pathway Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-013L $592.00
add to cart
Cignal RBP-Jk Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-014L $592.00
add to cart
Cignal NFAT Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-015L $592.00
add to cart
Cignal RARE Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-016L $592.00
add to cart
Cignal SMAD Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-017G $592.00
add to cart
Cignal SMAD Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-017L $592.00
add to cart
Cignal TCF/LEF Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-018G $592.00
add to cart
Cignal TCF/LEF Reporter Assay Kit (LUC)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-018L $592.00
add to cart
Cignal C/EBP Reporter (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-1001G $592.00
add to cart
Cignal RBP-Jk Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-1014G $592.00
add to cart
Cignal Androgen Receptor Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-1019L $592.00
add to cart
Cignal FOXO Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-1022L $592.00
add to cart
Cignal SP1 Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-1027G $592.00
add to cart
Cignal GATA Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-1035L $592.00
add to cart
Cignal Vitamin D Receptor Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-2029L $592.00
add to cart
Cignal ERSE Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-2032L $592.00
add to cart
Cignal XRE Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-2045L $592.00
add to cart
Cignal EGR1 Reporter Assay Kit (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-3021G $592.00
add to cart
Cignal PPAR Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-3026L $592.00
add to cart
Cignal HNF4 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-3039L $592.00
add to cart
Cignal Pax6 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-3042L $592.00
add to cart
Cignal Heat Shock Response Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-4023L $592.00
add to cart
Cignal KLF4 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-4036L $592.00
add to cart
Cignal Antioxidant Response Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-5020L $592.00
add to cart
Cignal MTF1 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-5033L $592.00
add to cart
Cignal FOXO Reporter (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-6022G $592.00
add to cart
Cignal SP1 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-6027L $592.00
add to cart
Cignal GLI Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-6030L $592.00
add to cart
Cignal PR Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-6043L $592.00
add to cart
Cignal MEF2 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-7024L $592.00
add to cart
Cignal Nanog Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-7037L $592.00
add to cart
Cignal IRF1 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-7040L $592.00
add to cart
Cignal XRE Reporter (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-7045G $592.00
add to cart
Cignal EGR1 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-8021L $592.00
add to cart
Cignal AARE Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-8034L $592.00
add to cart
Cignal STAT3 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-9028L $592.00
add to cart
Cignal ATF6 Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-9031L $592.00
add to cart
Cignal KLF4 Reporter (GFP)
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-9036G $592.00
add to cart
Cignal RXR Reporter (luc) Kit
For 500 reporter assays, 500 negative control assays, and 250 positive control assays; 500 µl (100 ng/µl) Reporter (inducible transcription-factor-responsive GFP reporter); 500 µl (100 ng/µl) Negative control (GFP reporter construct with GFP expression controlled by a minimal promoter); 250 µl (100 ng/µl) Positive control (constitutively expressing GFP construct)
show details 		336841 CCS-9044L $592.00
add to cart
Configure
Product Product no. Cat. no. List price:
 
 
Show details 		    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Available Controls
Configure
Product Product no. Cat. no. List price:
 
 
Show details 		    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.

Cignal Reporter Assay Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


0
TNFα activates NFkB signaling activity in a dose-dependent manner.
293 H cells were transfected with Cignal NFkB Reporter Assay, negative or positive control. After 24 hours, cells were treated with different doses of hTNFα for a further 24 hours. Dual-luciferase assays were performed, and reporter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and standard deviation is shown.
1
Assessing RNA interference phenotypes.

Cignal p53 Reporter Assays showed that p53 siRNA treatment abolished p53 transcription activity. HCT 116 cells were transfected with p53 reporter, negative control, and positive control, together with p53 siRNA or negative control siRNA. Dual-luciferase assays were performed. Reporter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.

2
Assessing overexpression phenotypes.

Cignal RBP-Jκ Reporter Assay showed upregulation of Notch signaling activity after overexpression of activated Notch1. 293 H cells were transfected with RBP-Jκ reporter, negative control, and positive control. After 24 hours, cells were infected with 100 MOI of recombinant adenoviruses expressing activated Notch1 (Ad-NICD) or 100 MOI of recombinant adenovirus expressing GFP (Ad-GFP) for another 18 hours. Dual-luciferase assays were performed. Reporter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.

3
Verification of small molecule drug candidate.

Cignal Retinoic Acid Response Element (RARE) Reporter Assay reported elevated retinoic acid receptor pathway activity after the treatment of all trans-retinoic acid (ATRA). CHO-K1 cells were transfected with RARE reporter, negative control, and positive control. After 16 hours, medium was changed to assay medium. After 24 hours, cells were treated with 1 µM all trans-rectinoic acid (ATRA) for 6 hours. Dual-luciferase assay was performed, and promoter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.

4
Cignal Reporter Assay shows that hTNFa activated NFkB signaling pathway.
293 H cells were transfected with the Cignal NFkB-GFP Reporter Assay or negative control. After 16 hours, medium was changed to assay medium. After 24 hours, cells were treated with 10 ng/ml TNFα. After 18 hours, medium was removed from the wells and fluorescence activity was measured using a fluorometer. The fluorescence activity present in treated and nontreated negative control wells was subtracted from the fluorescence activity in treated and nontreated Cignal NFkB-GFP Reporter Assay wells and relative fluorescence activities expressed as arbitrary units. Experiments were performed in triplicate and the standard deviations are shown.
5
Cignal Reporter Assay measures activation of serum response factor (SRF) transcription activity.

293 H cells were transfected with the Cignal SRF-GFP Reporter Assay or negative control. After 16 hours, medium was changed to assay medium. After 24 hours, cells were treated with 10 ng/ml PMA and 10% serum. After 18 hours, medium was removed from the wells and fluorescence activity was measured using a fluorometer. The fluorescence activity present in treated and nontreated negative control wells was subtracted from the fluorescence activity in treated and nontreated Cignal SRF-GFP Reporter Assay wells and relative fluorescence activities expressed as arbitrary units. Experiments were performed in triplicate and the standard deviations are shown.

6
Cignal Reporter Assay procedure.
7
Cignal Reporter Assay principle.
Performance
Cignal Reporter Assays may be used in many applications, such as functional genomics (see figures "Assessing RNA interference phenotypes" and "Assessing overexpression phenotypes"), functional proteomics (see figure "TNFα activates NFkB signaling activity in a dose-dependent manner"), drug discovery (see figure "Verification of small molecule drug candidate", and quantitative fluorometry (see figures "Cignal Reporter Assay shows that hTNFα activated NFkB signaling pathway" and "Cignal Reporter Assay measures activation of serum response factor (SRF) transcription activity").
Principle

Cignal Reporter Assays consist of multiple repeats of a specific transcription factor’s binding site and basic promoter elements to drive the expression of a reporter gene (firefly luciferase or GFP). These reporters are powerful tools in functional genomics and drug discovery for assessing pathway activity. When the pathway is activated or inhibited by a drug candidate, gene knockdown, overexpression, or peptide, luciferase or GFP reporter activity is modulated and can be measured quantitatively and rapidly (see figure "Cignal Reporter Assay principle"). The Cignal Reporter Assays are available as transfection-ready constructs utilizing two reporter systems: the dual-luciferase reporter or the GFP reporter (see flowchart "Cignal Reporter Assay procedure").

The dual-luciferase format is an end-point assay providing unsurpassed sensitivity, specificity, and signal-to-noise ratios. This format is available for all Cignal Reporter Assays. Each assay includes a pathway-focused transcription factor reporter and a noninducible negative control, as well as luciferase and GFP positive controls.

The GFP format is an outstanding method for monitoring live cell pathway regulation, with single cell resolution. It includes a pathway-focused transcription factor reporter and positive and negative controls.

Procedure

Cignal Reporter Assays include a preformulated, transfection-ready pathway reporter (dual-luciferase or GFP), plus a positive and negative control. The inducible pathway reporter and noninducible negative control are transfected and subjected to experimental treatments in parallel.

Dual-luciferase

Dual-luciferase results are calculated for each transfectant. The change in the activity of each signaling pathway is determined by comparing the normalized luciferase activities of the reporter in treated versus untreated transfectants. The identically treated negative control transfectants serve as a specificity control. The positive control serves as a control for transfection efficiency, by monitoring firefly luciferase and Renilla expression.

GFP

GFP expression is quantified using a flow cytometer, fluorescence microscope, or fluorometer. The change in the activity of each signaling pathway is determined by comparing the GFP activities in treated versus untreated transfectants. The identically treated negative control transfectants serve as a specificity control. The positive control serves as a control for transfection efficiency, by monitoring GFP expression from the constitutively expressing CMV-GFP reporter.

Applications

Cignal Reporter Assays are powerful tools in functional genomics, proteomics, and drug discovery for assessing the biological impact of pathway inhibition or activation with siRNAs, proteins, and small molecule compounds.

You are not authorized to download the resource

Brochures & Guides (1)
Cignal Reporter Assays
For cell-based analysis of pathway signaling activity
Show details
Kit Handbooks (1)
(EN) - Cignal Reporter Assay Handbook
For cell-based pathway activity assays
Show details
Transfection Protocols (2)
Transfection Cell Database
Search for transfection data by nucleic acid, cell line, and transfection reagent. Our database contains data from researchers like yourself who have shared their experimental results with us.
View
TransFect Protocol Database
Transfection protocols for specific cell types and plate formats that save you the time and effort of adapting existing protocols to fit your requirements. Simply select the cell type, nucleic acid, and culture format to receive a QIAGEN transfection protocol to print out or download in convenient PDF format.
View
fragment fix placeholder

Customers who bought these products also bought